Evidence map›Paper›PMID 37398004›Full record

ArticlebioRxiv : the preprint server for biology2023

Structural basis for flagellin induced NAIP5 activation.

Bhaskar Paidimuddala, Jianhao Cao, Liman Zhang

Open access · greenAbstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 1 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Bhaskar PaidimuddalaDepartment of Chemical Physiology and Biochemistry, Oregon Health and Science University, Portland, OR 97239, USA.
Jianhao CaoDepartment of Chemical Physiology and Biochemistry, Oregon Health and Science University, Portland, OR 97239, USA.
Liman ZhangDepartment of Chemical Physiology and Biochemistry, Oregon Health and Science University, Portland, OR 97239, USA.
Oregon Health & Science University · US

Funding

Pacific Northwest Center for Cryo-EM - Screening supplementU24GM129547 · NIGMS · OREGON HEALTH & SCIENCE UNIVERSITY · PI EVANS, JAMES E, GOUAUX, JAMES E · 2018 to 2023
$54.8M
Elucidating the Structural Mechanisms of NAIP Receptors in Bacterial Detection and Inflammasome ActivationR01AI165580 · NIAID · VAN ANDEL RESEARCH INSTITUTE · PI Liman Zhang · 2022 to 2026
$2.1M
NIAID NIH HHS R01 AI165580NIGMS NIH HHS U24 GM129547
6 · The paper itself

Abstract

The NAIP/NLRC4 inflammasome is activated when NAIP binds to a gram-negative bacterial ligand. Initially, NAIP exists in an inactive state with a wide-open conformation. Upon ligand binding, the winged helix domain (WHD) of NAIP is activated and forms steric clash with NLRC4 to open it up. However, how ligand binding induces the conformational change of NAIP is less clear. To understand this process, we investigated the dynamics of the ligand binding region of inactive NAIP5 and solved the cryo-EM structure of NAIP5 in complex with its specific ligand, FliC from flagellin, at 2.93 Å resolution. The structure revealed a "trap and lock" mechanism in FliC recognition, whereby FliC-D0

Indexed as

Bacteria infectioncryo-EMFlagellinNAIP/NLRC4 inflammasomeNAIP receptorNLR proteins

Identifiers

PMID37398004
PMCPMC10312664
OpenAlexW4380624185

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.