Evidence map›Paper›PMID 37397810›Full record

ArticleCurrent research in neurobiology2023

Efficient viral expression of a chemogenetic receptor in the old-world monkey amygdala.

Walter Lerchner, Kiana Dash, Deborah Rose, Mark A G Eldridge, Kathryn M Rothenhoefer, Xuefeng Yan, Vincent D Costa, Bruno Averbeck, Barry J Richmond

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Current research in neurobiology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Walter LerchnerLaboratory of Neuropsychology, National Institute of Mental Health, National Institutes of Health, Bethesda, MD, 20892, USA.
Kiana DashLaboratory of Neuropsychology, National Institute of Mental Health, National Institutes of Health, Bethesda, MD, 20892, USA.
Deborah RoseLaboratory of Neuropsychology, National Institute of Mental Health, National Institutes of Health, Bethesda, MD, 20892, USA.
Mark A G EldridgeLaboratory of Neuropsychology, National Institute of Mental Health, National Institutes of Health, Bethesda, MD, 20892, USA.
Kathryn M RothenhoeferDivision of Neuroscience, Oregon National Primate Research Center, Oregon Health and Science University, Beaverton, OR, USA.
Xuefeng YanMolecular Imaging Branch, National Institute of Mental Health, National Institutes of Health, Bethesda, MD, USA.
Vincent D CostaDivision of Neuroscience, Oregon National Primate Research Center, Oregon Health and Science University, Beaverton, OR, USA.
Bruno AverbeckLaboratory of Neuropsychology, National Institute of Mental Health, National Institutes of Health, Bethesda, MD, 20892, USA.
Barry J RichmondLaboratory of Neuropsychology, National Institute of Mental Health, National Institutes of Health, Bethesda, MD, 20892, USA.

Funding

Neural Mechanisms Of Motivation And RewardZIAMH002619 · NIMH · NATIONAL INSTITUTE OF MENTAL HEALTH · PI RICHMOND, BARRY J · 2009 to 2025
$21.3M
Intramural NIH HHS ZIA MH002619
6 · The paper itself

Abstract

Genetically encoded synthetic receptors, such as the chemogenetic and optogenetic proteins, are powerful tools for functional brain studies in animals. In the primate brain, with its comparatively large, intricate anatomical structures, it can be challenging to express transgenes, such as the hM4Di chemogenetic receptor, in a defined anatomical structure with high penetrance. Here, we compare parameters for lentivirus vector injections in the rhesus monkey amygdala. We find that four injections of 20 μl, infused at 0.5 μl/min, can achieve neuronal hM4Di expression in 50-100% of neurons within a 60 mm

Identifiers

PMID37397810
PMCPMC10313863

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.