Evidence map›Paper›PMID 37397630›Full record

ArticleCanadian journal of veterinary research = Revue canadienne de recherche veterinaire2023

Development of an indirect ELISA method based on the VP1 protein for detection of IgG antibodies against porcine sapelovirus.

Jiajia Liu, Benqiang Li, Jie Tao, Jinghua Cheng, Ying Shi, Changtao Qiao, Xiaohui Shen, Huili Liu

Open access · greenAbstract read
PubMed Full text at PMC
In one paragraph

Article in Canadian journal of veterinary research = Revue canadienne de recherche veterinaire, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.9field-weighted citation impact, top 24% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 4 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 1 institution in 1 country.

Jiajia Liu *Institute of Animal Husbandry and Veterinary Sciences, Shanghai Academy of Agricultural Sciences, Shanghai, 201106, PR China (J. Liu, Li, Tao, Cheng, Shi, Qiao, Shen, H. Liu); National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai, 201306, PR China (J. Liu).
Benqiang Li *Institute of Animal Husbandry and Veterinary Sciences, Shanghai Academy of Agricultural Sciences, Shanghai, 201106, PR China (J. Liu, Li, Tao, Cheng, Shi, Qiao, Shen, H. Liu); National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai, 201306, PR China (J. Liu).
Jie TaoInstitute of Animal Husbandry and Veterinary Sciences, Shanghai Academy of Agricultural Sciences, Shanghai, 201106, PR China (J. Liu, Li, Tao, Cheng, Shi, Qiao, Shen, H. Liu); National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai, 201306, PR China (J. Liu).
Jinghua ChengInstitute of Animal Husbandry and Veterinary Sciences, Shanghai Academy of Agricultural Sciences, Shanghai, 201106, PR China (J. Liu, Li, Tao, Cheng, Shi, Qiao, Shen, H. Liu); National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai, 201306, PR China (J. Liu).
Ying ShiInstitute of Animal Husbandry and Veterinary Sciences, Shanghai Academy of Agricultural Sciences, Shanghai, 201106, PR China (J. Liu, Li, Tao, Cheng, Shi, Qiao, Shen, H. Liu); National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai, 201306, PR China (J. Liu).
Changtao QiaoInstitute of Animal Husbandry and Veterinary Sciences, Shanghai Academy of Agricultural Sciences, Shanghai, 201106, PR China (J. Liu, Li, Tao, Cheng, Shi, Qiao, Shen, H. Liu); National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai, 201306, PR China (J. Liu).
Xiaohui ShenInstitute of Animal Husbandry and Veterinary Sciences, Shanghai Academy of Agricultural Sciences, Shanghai, 201106, PR China (J. Liu, Li, Tao, Cheng, Shi, Qiao, Shen, H. Liu); National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai, 201306, PR China (J. Liu).
Huili LiuInstitute of Animal Husbandry and Veterinary Sciences, Shanghai Academy of Agricultural Sciences, Shanghai, 201106, PR China (J. Liu, Li, Tao, Cheng, Shi, Qiao, Shen, H. Liu); National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai, 201306, PR China (J. Liu).
Shanghai Ocean University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Porcine sapelovirus (PSV) is a newly emerging enterovirus that is widely prevalent in China. Since there is no clinical serological testing for PSV, the objective of this study was to develop an indirect enzyme-linked immunosorbent assay (i-ELISA) for detection of PSV immunoglobulin G (IgG) antibody in pigs. A PSV strain, named SHPD202148, was first isolated from the fecal samples of piglets. Its structural protein, VP1, was prokaryotic-expressed in the pET expression system, followed by purification. Using the recombinant protein with reactogenicity as coating antigen, an i-ELISA, characterized by high sensitivity and specificity, had a detection limit at 1:12 800 dilution with a determined cutoff value of 0.352. Finally, field sera collected from different pig herds were tested in parallel by the serum neutralization (SN) test. The result showed that 126 samples were positive and 36 were negative, with an agreement of 97.0% in both cases. This i-ELISA can be used as an alternative serological test for detecting antibodies against PSV in blood serum.

Indexed as

Immunoglobulin GPicornaviridaeAnimalsAntibodies, ViralAntigens, ViralEnzyme-Linked Immunosorbent AssayRecombinant ProteinsSensitivity and SpecificitySwineAntibodies, ViralAntigens, ViralImmunoglobulin GRecombinant Proteins

Identifiers

PMID37397630
PMCPMC10291703
OpenAlexW4382918194

What OpenQuestion holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.