Evidence map›Paper›PMID 37396790›Full record

ArticlemicroPublication biology2023

mScarlet and split fluorophore mScarlet resources for plasmid-based CRISPR/Cas9 knock-in in

Gillian Witten, Ella DeMott, George Huang, Francis Zelasko, Bailey de Jesus, Chandi Mulchand, Liam Schuck, Stephen Pullman, Amelie Perez, Priya Mahableshwarkar and 5 more

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in microPublication biology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
0.9field-weighted citation impact, top 25% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 6 citations in OpenAlex.

  1. Article
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  5. Article
  6. Investigation of PAL-1 requirement inmicroPublication biology · 2023
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors at 1 institution in 1 country.

Gillian Witten *Glow Worms Stream, Freshman Research Initiative, College of Natural Sciences, The University of Texas at Austin, Austin, Texas, USA.
Ella DeMott *Glow Worms Stream, Freshman Research Initiative, College of Natural Sciences, The University of Texas at Austin, Austin, Texas, USA.
George HuangGlow Worms Stream, Freshman Research Initiative, College of Natural Sciences, The University of Texas at Austin, Austin, Texas, USA.
Francis ZelaskoGlow Worms Stream, Freshman Research Initiative, College of Natural Sciences, The University of Texas at Austin, Austin, Texas, USA.
Bailey de JesusGlow Worms Stream, Freshman Research Initiative, College of Natural Sciences, The University of Texas at Austin, Austin, Texas, USA.
Chandi MulchandGlow Worms Stream, Freshman Research Initiative, College of Natural Sciences, The University of Texas at Austin, Austin, Texas, USA.
Liam SchuckGlow Worms Stream, Freshman Research Initiative, College of Natural Sciences, The University of Texas at Austin, Austin, Texas, USA.
Stephen PullmanGlow Worms Stream, Freshman Research Initiative, College of Natural Sciences, The University of Texas at Austin, Austin, Texas, USA.
Amelie PerezGlow Worms Stream, Freshman Research Initiative, College of Natural Sciences, The University of Texas at Austin, Austin, Texas, USA.
Priya MahableshwarkarGlow Worms Stream, Freshman Research Initiative, College of Natural Sciences, The University of Texas at Austin, Austin, Texas, USA.
Zheng WuDepartment of Neuroscience, The University of Texas at Austin, Austin, Texas, USA.
Eric Andrew CardonaDepartment of Neuroscience, The University of Texas at Austin, Austin, Texas, USA.
Jonathan T PierceDepartment of Neuroscience, The University of Texas at Austin, Austin, Texas, USA.
Daniel J DickinsonDepartment of Molecular Biosciences, The University of Texas at Austin, Austin, Texas, USA.
Ryan DoonanGlow Worms Stream, Freshman Research Initiative, College of Natural Sciences, The University of Texas at Austin, Austin, Texas, USA.
The University of Texas at Austin · US

Funding

Synergistic patterned neurodegeneration by APP and APOE4RF1AG057355 · NIA · UNIVERSITY OF TEXAS AT AUSTIN · PI PIERCE, JONATHAN THOMAS · 2017 to 2017
$1.9M
High-throughput interrogation of autism risk genes: from molecules to behaviorR01MH133243 · NIMH · UNIVERSITY OF TEXAS AT AUSTIN · PI PIERCE, JONATHAN THOMAS · 2023 to 2025
$1.1M
Systematic functional study of 21st chromosome ortholog overexpression in C. elegansR21OD032463 · OD · UNIVERSITY OF TEXAS AT AUSTIN · PI PIERCE, JONATHAN THOMAS · 2022 to 2023
$574k
NIA NIH HHS RF1 AG057355NIH HHS R21 OD032463NIMH NIH HHS R01 MH133243
6 · The paper itself

Abstract

Fluorescent proteins allow the expression of a gene and the behavior of its protein product to be observed in living animals. The ability to create endogenous fluorescent protein tags via CRISPR genome engineering has revolutionized the authenticity of this expression, and mScarlet is currently our first-choice red fluorescent protein (RFP) for visualizing gene expression

Identifiers

PMID37396790
PMCPMC10308244
OpenAlexW4382919159

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.