Evidence map›Paper›PMID 37392244›Full record

ArticleEuropean journal of nutrition2023

Leucine and arginine enhance milk fat and milk protein synthesis via the CaSR/G

Qihui Li, Jiaming Chen, Jiaxin Liu, Tongbin Lin, Xinghong Liu, Shuchang Zhang, Xianhuai Yue, Xiaoli Zhang, Xiangfang Zeng, Man Ren and 2 more

Abstract read
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In one paragraph

Article in European journal of nutrition, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
1.3field-weighted citation impact, top 20% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 4 citations in OpenAlex.

  1. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 3 institutions in 1 country.

Qihui LiGuangdong Province Key Laboratory of Animal Nutrition Control, College of Animal Science, South China Agricultural University, Guangzhou, 510642, China.
Jiaming ChenGuangdong Province Key Laboratory of Animal Nutrition Control, College of Animal Science, South China Agricultural University, Guangzhou, 510642, China.
Jiaxin LiuGuangdong Province Key Laboratory of Animal Nutrition Control, College of Animal Science, South China Agricultural University, Guangzhou, 510642, China.
Tongbin LinGuangdong Province Key Laboratory of Animal Nutrition Control, College of Animal Science, South China Agricultural University, Guangzhou, 510642, China.
Xinghong LiuGuangdong Province Key Laboratory of Animal Nutrition Control, College of Animal Science, South China Agricultural University, Guangzhou, 510642, China.
Shuchang ZhangGuangdong Province Key Laboratory of Animal Nutrition Control, College of Animal Science, South China Agricultural University, Guangzhou, 510642, China.
Xianhuai YueGuangdong Province Key Laboratory of Animal Nutrition Control, College of Animal Science, South China Agricultural University, Guangzhou, 510642, China.
Xiaoli ZhangGuangdong Province Key Laboratory of Animal Nutrition Control, College of Animal Science, South China Agricultural University, Guangzhou, 510642, China.
Xiangfang ZengState Key Laboratory of Animal Nutrition, Ministry of Agriculture and Rural Affairs Feed Industry Center, China Agricultural University, Beijing, China.
Man RenAnhui Provincial Key Laboratory of Animal Nutritional Regulation and Health, College of Animal Science, Anhui Science and Technology University, Fengyang, China.
Wutai GuanGuangdong Province Key Laboratory of Animal Nutrition Control, College of Animal Science, South China Agricultural University, Guangzhou, 510642, China.
Shihai ZhangGuangdong Province Key Laboratory of Animal Nutrition Control, College of Animal Science, South China Agricultural University, Guangzhou, 510642, China. zhangshihai@scau.edu.cn.
South China Agricultural University · CNAnhui Science and Technology University · CNMinistry of Agriculture and Rural Affairs · CN

Funding

Anhui Province Key Laboratory of Animal Nutrition Regulation and Health No. APKLANRH202105Guangdong Basic and Applied Basic Research Foundation No.2021A1515010440Guangdong Basic and Applied Basic Research Foundation No.2023A1515012098National Key R&D Program of China No.2021YFD1300700National Natural Science Foundation of China No.31802067National Natural Science Foundation of China No.31872364Science and Technology Program of Guangzhou No.202102020056
6 · The paper itself

Abstract

BACKGROUND AND

aimsAmino acids (AAs) not only constitute milk protein but also stimulate milk synthesis through the activation of mTORC1 signaling, but which amino acids that have the greatest impact on milk fat and protein synthesis is still very limited. In this study, we aimed to identify the most critical AAs involved in the regulation of milk synthesis and clarify how these AAs regulate milk synthesis through the G-protein-coupled receptors (GPCRs) signaling pathway.

methodsIn this study, a mouse mammary epithelial cell line (HC11) and porcine mammary epithelial cells (PMECs) were selected as study subjects. After treatment with different AAs, the amount of milk protein and milk fat synthesis were detected. Activation of mTORC1 and GPCRs signaling induced by AAs was also investigated.

resultsIn this study, we demonstrate that essential amino acids (EAAs) are crucial to promote lactation by increasing the expression of genes and proteins related to milk synthesis, such as ACACA, FABP4, DGAT1, SREBP1, α-casein, β-casein, and WAP in HC11 cells and PMECs. In addition to activating mTORC1, EAAs uniquely regulate the expression of calcium-sensing receptor (CaSR) among all amino-acid-responsive GPCRs, which indicates a potential link between CaSR and the mTORC1 pathway in mammary gland epithelial cells. Compared with other EAAs, leucine and arginine had the greatest capacity to trigger GPCRs (p-ERK) and mTORC1 (p-S6K1) signaling in HC11 cells. In addition, CaSR and its downstream G proteins G

conclusionWe found that the G-protein-coupled receptor CaSR is an important amino acid sensor in mammary epithelial cells. Leucine and arginine promote milk synthesis partially through the CaSR/G

Indexed as

Milk ProteinsReceptors, Calcium-SensingAmino AcidsAnimalsArginineCaseinsEpithelial CellsFemaleLeucineMammary Glands, AnimalMechanistic Target of Rapamycin Complex 1MiceReceptors, G-Protein-CoupledSwineAmino AcidsArginineCaseinsLeucineMechanistic Target of Rapamycin Complex 1Milk ProteinsReceptors, Calcium-SensingReceptors, G-Protein-CoupledArginineCaSRG iG qLeucineMilk synthesis

Identifiers

PMID37392244
OpenAlexW4382795188

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.