ArticleBMC plant biology2023
The revealing of a novel double bond reductase related to perilla ketone biosynthesis in Perilla frutescens.
Article in BMC plant biology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
9 citing papers in PubMed, 12 citations in OpenAlex.
- Determinants of substrate specificity in mint pulegone reductase, a double bond reductase of the medium-chain dehydrogenase/reductase superfamily.The Journal of biological chemistry · 2026Article
- Study on biosynthesis pathway and accumulation mechanism of the dihydrochalcones inHorticulture research · 2026Article
- Genome-Wide Identification and Analysis of BAHD Acyltransferases Involved in Anthocyanin Biosynthesis inBiology · 2026Article
- Pharmacological effects, classification, genetic and molecular studies of different chemotypes essential oil ofJournal of pharmaceutical analysis · 2026Review
- Functional Characterization of Double-Bond Reductases in Dihydro-β-Ionone Biosynthesis inPlants (Basel, Switzerland) · 2025Article
- Genome-wide analysis of PfBBXs transcription factors in Perilla frutescens and their expression responses to different light intensities.Plant molecular biology · 2025Article
- Comprehensive Review ofFoods (Basel, Switzerland) · 2025Review
- Rapid discrimination and quantification of chemotypes in Perillae folium using FT-NIR spectroscopy and GC-MS combined with chemometrics.Food chemistry: X · 2024Article
- Critical radicle length window governing loss of dehydration tolerance in germinated Perilla seeds: insights from physiological and transcriptomic analyses.BMC plant biology · 2024Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
backgroundPerilla frutescens is widely used as both a medicine and a food worldwide. Its volatile oils are its active ingredients, and, based on the different volatile constituents, P. frutescens can be divided into several chemotypes, with perilla ketone (PK) being the most common. However, the key genes involved in PK biosynthesis have not yet been identified.
resultsIn this study, metabolite constituents and transcriptomic data were compared in leaves of different levels. The variation in PK levels was the opposite of that of isoegoma ketone and egoma ketone in leaves at different levels. Based on transcriptome data, eight candidate genes were identified and successfully expressed in a prokaryotic system. Sequence analysis revealed them to be double bond reductases (PfDBRs), which are members of the NADPH-dependent, medium-chain dehydrogenase/reductase (MDR) superfamily. They catalyze the conversion of isoegoma ketone and egoma ketone into PK in in vitro enzymatic assays. PfDBRs also showed activity on pulegone, 3-nonen-2-one, and 4-hydroxybenzalacetone. In addition, several genes and transcription factors were predicted to be associated with monoterpenoid biosynthesis, and their expression profiles were positively correlated with variations in PK abundance, suggesting their potential functions in PK biosynthesis.
conclusionsThe eight candidate genes encoding a novel double bond reductase related to perilla ketone biosynthesis were identified in P. frutescens, which carries similar sequences and molecular features as the MpPR and NtPR from Nepeta tenuifolia and Mentha piperita, respectively. These findings not only reveal the pivotal roles of PfDBR in exploring and interpreting PK biological pathway but also contribute to facilitating future studies on this DBR protein family.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.