ArticlePLoS pathogens2023
High-throughput super-resolution analysis of influenza virus pleomorphism reveals insights into viral spatial organization.
Article in PLoS pathogens, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 29 citations in OpenAlex.
- Unsupervised classification of influenza virus surface spikes from cryo-EM reconstructions using head-to-stem width ratio.Virology journal · 2025Article
- High-throughput single-virion DNA-PAINT reveals structural diversity, cooperativity, and flexibility during selective packaging in influenza.Nucleic acids research · 2025Article
- Implications of morphological variation in influenza viruses.Microbiology and molecular biology reviews : MMBR · 2025Review
- A Broad Wildlife Survey of Influenza A Virus in the Orinoco Flooded Savannas from Colombia: New Reports and Perspectives.Animals : an open access journal from MDPI · 2025Article
- Single influenza A viruses induce nanoscale cellular reprogramming at the virus-cell interface.Nature communications · 2025Article
- Influenza A virus rapidly adapts particle shape to environmental pressures.Nature microbiology · 2025Article
- The nanoscale organization of the Nipah virus fusion protein informs new membrane fusion mechanisms.eLife · 2025Article
- Purification of Influenza Virions.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Resolving viral structural complexity by super-resolution microscopy.Archives of virology · 2024Review
- Advantages of Broad-Spectrum Influenza mRNA Vaccines and Their Impact on Pulmonary Influenza.Vaccines · 2024Review
- Resolving the differential distribution of structural proteins in baculovirus using single-molecule localization microscopy.The Journal of general virology · 2024Article
- Single-molecule detection and super-resolution imaging with a portable and adaptable 3D-printed microscopy platform (Brick-MIC).Science advances · 2024Article
- Single-molecule detection and super-resolution imaging with a portable and adaptable 3D-printed microscopy platform (Brick-MIC).bioRxiv : the preprint server for biology · 2024Article
- Optimized production and fluorescent labeling of SARS-CoV-2 virus-like particles.Scientific reports · 2022Article
- Virus morphology: Insights from super-resolution fluorescence microscopy.Biochimica et biophysica acta. Molecular basis of disease · 2022Review
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
Abstract
Many viruses form highly pleomorphic particles. In influenza, virion structure is of interest not only in the context of virus assembly, but also because pleomorphic variations may correlate with infectivity and pathogenicity. We have used fluorescence super-resolution microscopy combined with a rapid automated analysis pipeline, a method well-suited to the study of large numbers of pleomorphic structures, to image many thousands of individual influenza virions; gaining information on their size, morphology and the distribution of membrane-embedded and internal proteins. We observed broad phenotypic variability in filament size, and Fourier transform analysis of super-resolution images demonstrated no generalized common spatial frequency patterning of HA or NA on the virion surface, suggesting a model of virus particle assembly where the release of progeny filaments from cells occurs in a stochastic way. We also showed that viral RNP complexes are located preferentially within Archetti bodies when these were observed at filament ends, suggesting that these structures may play a role in virus transmission. Our approach therefore offers exciting new insights into influenza virus morphology and represents a powerful technique that is easily extendable to the study of pleomorphism in other pathogenic viruses.
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Registered trials
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