Evidence map›Paper›PMID 37388682›Full record

ArticleJACS Au2023

Deuterium- and Alkyne-Based Bioorthogonal Raman Probes for In Situ Quantitative Metabolic Imaging of Lipids within Plants.

Simon Sau Yin Law, Masato Asanuma, Jingwen Shou, Yasuyuki Ozeki, Yutaka Kodama, Keiji Numata

Abstract read
In one paragraph

Article in JACS Au, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed.

  1. Review
  2. Raman imaging of molecular groups in the wavenumber silent region.Analytical and bioanalytical chemistry · 2026
    Review
  3. Review
  4. Review
  5. Review
  6. Synthetic Lipid Biology.Chemical reviews · 2025
    Review
  7. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Simon Sau Yin LawBiomacromolecules Research Team, RIKEN Center for Sustainable Resource Science, Wako, Saitama 351-0198, Japan.ORCID https://orcid.org/0000-0002-9650-2958
Masato AsanumaGraduate School of Engineering, University of Tokyo, Bunkyo-ku, Tokyo 113-8656, Japan.
Jingwen ShouGraduate School of Engineering, University of Tokyo, Bunkyo-ku, Tokyo 113-8656, Japan.
Yasuyuki OzekiGraduate School of Engineering, University of Tokyo, Bunkyo-ku, Tokyo 113-8656, Japan.ORCID https://orcid.org/0000-0002-9004-0799
Yutaka KodamaBiomacromolecules Research Team, RIKEN Center for Sustainable Resource Science, Wako, Saitama 351-0198, Japan.ORCID https://orcid.org/0000-0002-4720-7311
Keiji NumataBiomacromolecules Research Team, RIKEN Center for Sustainable Resource Science, Wako, Saitama 351-0198, Japan.ORCID https://orcid.org/0000-0003-2199-7420

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Plants can rapidly respond to different stresses by activating multiple signaling and defense pathways. The ability to directly visualize and quantify these pathways in real time using bioorthogonal probes would have practical applications, including characterizing plant responses to both abiotic and biotic stress. Fluorescence-based labels are widely used for tagging of small biomolecules but are relatively bulky and with potential effects on their endogenous localization and metabolism. This work describes the use of deuterium- and alkyne-derived fatty acid Raman probes to visualize and track the real-time response of plants to abiotic stress within the roots. Relative quantification of the respective signals could be used to track their localization and overall real-time responses in their fatty acid pools due to drought and heat stress without labor-intensive isolation procedures. Their overall usability and low toxicity suggest that Raman probes have great untapped potential in the field of plant bioengineering.

Identifiers

PMID37388682
PMCPMC10302745

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.