Evidence map›Paper›PMID 37386941›Full record

ArticleStem cells (Dayton, Ohio)2023

Serum Response Factor Reduces Gene Expression Noise and Confers Cell State Stability.

Jian Zhang, Qiao Wu, Xiao Hu, Yadong Wang, Jun Lu, Raja Chakraborty, Kathleen A Martin, Shangqin Guo

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Stem cells (Dayton, Ohio), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.5field-weighted citation impact, top 33% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 3 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 1 country.

Jian ZhangDepartment of Cell Biology, Yale University, New Haven, CT, USA.ORCID 0000-0002-0790-4462
Qiao WuDepartment of Cell Biology, Yale University, New Haven, CT, USA.
Xiao HuDepartment of Cell Biology, Yale University, New Haven, CT, USA.
Yadong WangYale Stem Cell Center, Yale University, New Haven, CT, USA.
Jun LuYale Stem Cell Center, Yale University, New Haven, CT, USA.ORCID 0000-0003-2726-7613
Raja ChakrabortyDepartment of Medicine, Section of Cardiovascular Medicine, Yale University, New Haven, CT, USA.
Kathleen A MartinDepartment of Medicine, Section of Cardiovascular Medicine, Yale University, New Haven, CT, USA.
Shangqin GuoDepartment of Cell Biology, Yale University, New Haven, CT, USA.ORCID 0000-0003-1157-0423
Yale Cancer Center · USYale University · US

Funding

Yale Liver CenterP30DK034989 · NIDDK · YALE UNIVERSITY · PI YASUKO IWAKIRI · 1986 to 2026
$31.1M
Yale Cooperative Hematology Specialized Core CenterU54DK106857 · NIDDK · YALE UNIVERSITY · PI JOHN HWA, Diane S Krause · 2015 to 2026
$9.7M
Molecular definition of cancer cell-of-originDP2GM123507 · NIGMS · YALE UNIVERSITY · PI GUO, SHANGQIN · 2016 to 2016
$2.5M
NIDDK NIH HHS P30 DK034989NIDDK NIH HHS U54 DK106857NIGMS NIH HHS DP2 GM123507
6 · The paper itself

Abstract

The role of serum response factor (Srf), a central mediator of actin dynamics and mechanical signaling, in cell identity regulation is debated to be either a stabilizer or a destabilizer. We investigated the role of Srf in cell fate stability using mouse pluripotent stem cells. Despite the fact that serum-containing cultures yield heterogeneous gene expression, deletion of Srf in mouse pluripotent stem cells leads to further exacerbated cell state heterogeneity. The exaggerated heterogeneity is detectible not only as increased lineage priming but also as the developmentally earlier 2C-like cell state. Thus, pluripotent cells explore more variety of cellular states in both directions of development surrounding naïve pluripotency, a behavior that is constrained by Srf. These results support that Srf functions as a cell state stabilizer, providing rationale for its functional modulation in cell fate intervention and engineering.

Indexed as

Pluripotent Stem CellsSerum Response FactorActinsAnimalsCell DifferentiationGene ExpressionMiceActinsSerum Response FactorCell identityHeterogeneityPluripotent stem cellsSrf

Identifiers

PMID37386941
PMCPMC11009695
OpenAlexW4382598203

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.