Evidence map›Paper›PMID 37386758›Full record

Observational studyCancer control : journal of the Moffitt Cancer Center

Detection of Nine Oncogenes Amplification in Lung and Colorectal Cancer Formalin-Fixed Paraffin-Embedded Tissue Samples using Combined Next-Generation Sequencing-Based Script and Digital Droplet Polymerase Chain Reaction.

Christophe Bontoux, Alice Guyard, Audrey Lupo, Karim Diallo, Khedidja Rebah, Agathe Hercent, Edouard Guenzi, Jérome Cros, Jérome Lamoril, Karen Leroy and 1 more

Open access · goldAbstract readObservational Study
PubMed Full text at PMC Publisher
In one paragraph

Observational study in Cancer control : journal of the Moffitt Cancer Center. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.5field-weighted citation impact, top 27% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 2 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 4 institutions in 1 country.

Christophe BontouxLaboratory of Clinical and Experimental Pathology, Centre Hospitalier Universitaire de Nice, Université Côte d'Azur, Hospital-Integrated Biobank, Hôpital Pasteur, Nice, France.ORCID 0000-0001-5446-6197
Alice GuyardDepartment of Genetics, University Hospital Bichat-Claude Bernard, AP-HP, Paris University, Paris, France.
Audrey LupoDepartment of Pathology, University Hospital Bichat-Claude Bernard, AP-HP, University Paris-Diderot, Paris, France.
Karim DialloLaboratory of Clinical and Experimental Pathology, Centre Hospitalier Universitaire de Nice, Université Côte d'Azur, Hospital-Integrated Biobank, Hôpital Pasteur, Nice, France.
Khedidja RebahLaboratory of Clinical and Experimental Pathology, Centre Hospitalier Universitaire de Nice, Université Côte d'Azur, Hospital-Integrated Biobank, Hôpital Pasteur, Nice, France.
Agathe HercentLaboratory of Clinical and Experimental Pathology, Centre Hospitalier Universitaire de Nice, Université Côte d'Azur, Hospital-Integrated Biobank, Hôpital Pasteur, Nice, France.
Edouard GuenziLaboratory of Clinical and Experimental Pathology, Centre Hospitalier Universitaire de Nice, Université Côte d'Azur, Hospital-Integrated Biobank, Hôpital Pasteur, Nice, France.
Jérome CrosDepartment of Pathology, Hôpitaux Universitaire Paris Centre, Hôpital Cochin, AP-HP, Université de Paris, Paris, France.
Jérome LamorilLaboratory of Clinical and Experimental Pathology, Centre Hospitalier Universitaire de Nice, Université Côte d'Azur, Hospital-Integrated Biobank, Hôpital Pasteur, Nice, France.
Karen LeroyDepartment of Pathology, University Hospital Beaujon, AP-HP, University Paris-Diderot, Paris, France.
Nathalie Theou-AntonLaboratory of Clinical and Experimental Pathology, Centre Hospitalier Universitaire de Nice, Université Côte d'Azur, Hospital-Integrated Biobank, Hôpital Pasteur, Nice, France.
Université Côte d'Azur · FRUniversité Claude Bernard Lyon 1 · FRDélégation Paris 7 · FRUniversité Paris Cité · FR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

introductionGene copy number variations have theranostic impact and require reliable methods for their identification. We aimed to evaluate the reliability of combined next-generation sequencing (NGS) and digital droplet PCR (ddPCR) method for gene amplification evaluation.

methodsWe conducted a retrospective multicentric observational study.

results55 patients (9 control, 25

conclusionCombined NGS-based script and ddPCR method is reliable and easily feasible for the detection of gene amplifications, providing useful data for guided therapy in cancer.

Indexed as

Colorectal NeoplasmsDNA Copy Number VariationsFormaldehydeHigh-Throughput Nucleotide SequencingHumansIn Situ Hybridization, FluorescenceLungOncogenesParaffin EmbeddingPolymerase Chain ReactionReproducibility of ResultsRetrospective StudiesFormaldehydeamplificationbioinformaticscolorectal cancercopy numberdigital droplet PCRgeneslung cancersequencing data

Identifiers

PMID37386758
PMCPMC10328004
OpenAlexW4382631805

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.