Evidence map›Paper›PMID 37385615›Full record

ArticleZhong nan da xue xue bao. Yi xue ban = Journal of Central South University. Medical sciences2023

MASH1 induces neuron transdifferentiation of adrenal medulla chromaffin cells.

Emin Peng, Chengping Hu, Juntao Feng, Ruoxi He

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Zhong nan da xue xue bao. Yi xue ban = Journal of Central South University. Medical sciences, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.6field-weighted citation impact, top 24% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 2 citations in OpenAlex.

  1. New Concepts of Regeneration and Renewal of Adrenal Chromaffin Cells.International journal of molecular sciences · 2025
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 2 countries.

Emin PengOutpatient Clinic of Xiangya Hospital International Medical Center, Central South University, Changsha 410008. 51318029@qq.com.
Chengping HuDepartment of Respiratory Medicine, National Clinical Key Specialty of Respiratory Disease, Branch of National Clinical Research Center for Respiratory Disease, Xiangya Hospital, Central South University, Changsha 410008.
Juntao FengDepartment of Respiratory Medicine, National Clinical Key Specialty of Respiratory Disease, Branch of National Clinical Research Center for Respiratory Disease, Xiangya Hospital, Central South University, Changsha 410008.
Ruoxi HeDepartment of Respiratory Medicine, National Clinical Key Specialty of Respiratory Disease, Branch of National Clinical Research Center for Respiratory Disease, Xiangya Hospital, Central South University, Changsha 410008. heruoxi@csu.edu.cn.
Central South University · CN

Funding

the National Natural Science Foundation 81400022the Natural Science Foundation of Hunan Province 2020JJ5897 and 2020JJ4904
6 · The paper itself

Abstract

objectivesNerve growth factor (NGF) induces neuron transdifferentiation of adrenal medulla chromaffin cells (AMCCs) and consequently downregulates the secretion of epinephrine (EPI), which may be involved in the pathogenesis of bronchial asthma. Mammalian achaete scute-homologous 1 (MASH1), a key regulator of neurogenesis in the nervous system, has been proved to be elevated in AMCCs with neuron transdifferentiation in vivo. This study aims to explore the role of MASH1 in the process of neuron transdifferentiation of AMCCs and the mechanisms.

methodsRat AMCCs were isolated and cultured. AMCCs were transfected with siMASH1 or MASH1 overexpression plasmid, then were stimulated with NGF and/or dexamethasone, PD98059 (a MAPK kinase-1 inhibitor) for 48 hours. Morphological changes were observed using light and electron microscope. Phenylethanolamine-N-methyltransferase (PNMT, the key enzyme for epinephrine synthesis) and tyrosine hydroxylase were detected by immunofluorescence. Western blotting was used to test the protein levels of PNMT, MASH1, peripherin (neuronal markers), extracellular regulated protein kinases (ERK), phosphorylated extracellular regulated protein kinases (pERK), and JMJD3. Real-time RT-PCR was applied to analyze the mRNA levels of

resultsCells with both tyrosine hydroxylase and PNMT positive by immunofluorescence were proved to be AMCCs. Exposure to NGF, AMCCs exhibited neurite-like processes concomitant with increases in pERK/ERK, peripherin, and MASH1 levels (all

conclusionsMASH1 is the key factor in neuron transdifferentiation of AMCCs. NGF-induced neuron transdifferentiation is probably mediated via pERK/MASH1 signaling.

Indexed as

Adrenal MedullaChromaffin CellsAnimalsBasic Helix-Loop-Helix ProteinsCell TransdifferentiationDexamethasoneEpinephrineMammalsNerve Growth FactorNeuronsPeripherinsProtein KinasesRatsTyrosine 3-MonooxygenaseAscl1 protein, ratBasic Helix-Loop-Helix ProteinsDexamethasoneEpinephrineNerve Growth FactorPeripherinsProtein KinasesTyrosine 3-Monooxygenasechromaffin cellsdexamethasoneMASH1nerve growth factorneurontransdifferentiation

Identifiers

PMID37385615
PMCPMC10930259
OpenAlexW4382632491

What OpenQuestion holds

Textmetadata
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.