Evidence map›Paper›PMID 37384350›Full record

ArticleSensors and actuators. B, Chemical2023

Label-free detection of synthetic, full genomic length HIV-1 RNA at the few-copy level.

Olivia O Dickens, Inayat Bajwa, Kelly Garcia-Ramos, Yeonjoon Suh, Chengyu Wen, Annie Cheng, Victoria Fethke, Yanji Yi, Ronald G Collman, A T Charlie Johnson

Abstract read
In one paragraph

Article in Sensors and actuators. B, Chemical, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Spherical SnOMikrochimica acta · 2025
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Olivia O DickensGraduate Group in Biochemistry and Molecular Biophysics, University of Pennsylvania, Philadelphia PA 19104.
Inayat BajwaDepartment of Bioengineering, University of Pennsylvania, Philadelphia PA 19104.
Kelly Garcia-RamosDepartment of Physics and Astronomy, University of Pennsylvania, Philadelphia PA 19104.
Yeonjoon SuhDepartment of Electrical and Systems Engineering, University of Pennsylvania, Philadelphia PA 19104.
Chengyu WenDepartment of Electrical and Systems Engineering, University of Pennsylvania, Philadelphia PA 19104.
Annie ChengDepartment of Physics and Astronomy, University of Pennsylvania, Philadelphia PA 19104.
Victoria FethkeDepartment of Physics and Astronomy, University of Pennsylvania, Philadelphia PA 19104.
Yanji YiDepartment of Medicine, Perelman School of Medicine, University of Pennsylvania, Philadelphia PA 19104.
Ronald G CollmanDepartment of Medicine, Perelman School of Medicine, University of Pennsylvania, Philadelphia PA 19104.
A T Charlie JohnsonGraduate Group in Biochemistry and Molecular Biophysics, University of Pennsylvania, Philadelphia PA 19104.

Funding

Virus & Reservoirs CoreP30AI045008 · NIAID · UNIVERSITY OF PENNSYLVANIA · PI ROBERT GROSS · 1999 to 2026
$78.6M
Epigenetic HIV Silencing in MacrophagesR33AI133696 · NIAID · UNIVERSITY OF PENNSYLVANIA · PI COLLMAN, RONALD G, LIEBERMAN, PAUL M. · 2020 to 2021
$1.7M
Nanomagnetic isolation and sensing for mobile HIV-1 self-testingR33AI147406 · NIAID · UNIVERSITY OF PENNSYLVANIA · PI ISSADORE, DAVID AARON · 2022 to 2023
$1.6M
Nanomagnetic isolation and sensing for mobile HIV-1 self-testingR61AI147406 · NIAID · UNIVERSITY OF PENNSYLVANIA · PI ISSADORE, DAVID AARON · 2019 to 2021
$1.4M
NIAID NIH HHS P30 AI045008NIAID NIH HHS R33 AI133696NIAID NIH HHS R33 AI147406NIAID NIH HHS R61 AI147406
6 · The paper itself

Abstract

Oligonucleotide-functionalized graphene biosensors show immense promise for use as label-free point of care devices for detection of nucleic acid biomarkers at clinically relevant levels. Graphene-based nucleic acid sensors can be fabricated at low cost and have been shown to reach limits of detection in the attomolar range. Here we demonstrate devices functionalized with 22mer or 8omer DNA probes are capable of detecting full length genomic HIV-1 subtype B RNA, with a limit of detection below 1 aM in nuclease free water. We also show that these sensors are suitable for detection directly in Qiazol lysis reagent, again with a limit of detection below 1 aM for both 22mer and 8omer probes.

Indexed as

AttomolarBiosensorsGrapheneNucleic Acid DetectionViral RNA

Identifiers

PMID37384350
PMCPMC10299804

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.