ArticlePlant biotechnology journal2023
Efficient proteome-wide identification of transcription factors targeting Glu-1: A case study for functional validation of TaB3-2A1 in wheat.
Article in Plant biotechnology journal, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 26 citations in OpenAlex.
- ROS-Centered Transcriptomic Regulatory Networks Linking Salinity Stress, Antioxidant Defense and Processability Traits inCurrent issues in molecular biology · 2026Review
- Genome-wide characterization and adaptive evolution of favorable gibberellin 2-oxidase alleles contributing to wheat agronomic traits.Journal of advanced research · 2026Article
- Cereal protein biofortification at the interface of nutrition, yield and sustainability.Nature plants · 2026Review
- Improving End-Use Quality Through Introducing Cysteines Into the Central Repeat Domain of High-Molecular-Weight Glutenin Subunit 1Dx2 in Bread Wheat.Plant biotechnology journal · 2026Article
- Identification of a cytokinin-responsive core promoter and interacting transcription factors of MtCKX1 in Medicago truncatula.BMC plant biology · 2026Article
- Altered starch composition and improved bread quality in novel wheat mutant reveal key transcriptional regulators.Current research in food science · 2026Article
- The TaMYB44-TaMYB1 module regulates grain amylose biosynthesis and flour viscosity in wheat.Plant communications · 2025Article
- Map-based cloning of ZmSS5, construction and validation of its regulatory pathway for maize kernel weight.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2025Article
- A large-scale gene regulatory network for rice endosperm starch biosynthesis and its application in genetic improvement of rice quality.Plant biotechnology journal · 2025Article
- Genome-wide characterization of GRF-GIF transcriptional modules in kenaf (Hibiscus cannabinus L.) reveals their roles in plant development and multi-stress adaptation.Functional & integrative genomics · 2025Article
- TaDL interacts with TaB3 and TaNF-YB1 to synergistically regulate the starch synthesis and grain quality in bread wheat.Journal of integrative plant biology · 2025Article
- Transcription Factor and Protein Regulatory Network ofPlants (Basel, Switzerland) · 2024Article
- Deciphering the Transcriptional Regulatory Network Governing Starch and Storage Protein Biosynthesis in Wheat for Breeding Improvement.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- A Functional InDel in the WRKY10 Promoter Controls the Degree of Flesh Red Pigmentation in Apple.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Effects of high-molecular-weight glutenin subunit on hard-steamed bread quality.Frontiers in genetics · 2024Article
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Authors and funding
15 authors at 4 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
High-molecular-weight glutenin subunits (HMW-GS), a major component of seed storage proteins (SSP) in wheat, largely determine processing quality. HMW-GS encoded by GLU-1 loci are mainly controlled at the transcriptional level by interactions between cis-elements and transcription factors (TFs). We previously identified a conserved cis-regulatory module CCRM1-1 as the most essential cis-element for Glu-1 endosperm-specific high expression. However, the TFs targeting CCRM1-1 remained unknown. Here, we built the first DNA pull-down plus liquid chromatography-mass spectrometry platform in wheat and identified 31 TFs interacting with CCRM1-1. TaB3-2A1 as proof of concept was confirmed to bind to CCRM1-1 by yeast one hybrid and electrophoretic mobility shift assays. Transactivation experiments demonstrated that TaB3-2A1 repressed CCRM1-1-driven transcription activity. TaB3-2A1 overexpression significantly reduced HMW-GS and other SSP, but enhanced starch content. Transcriptome analyses confirmed that enhanced expression of TaB3-2A1 down-regulated SSP genes and up-regulated starch synthesis-related genes, such as TaAGPL3, TaAGPS2, TaGBSSI, TaSUS1 and TaSUS5, suggesting that it is an integrator modulating the balance of carbon and nitrogen metabolism. TaB3-2A1 also had significant effects on agronomic traits, including heading date, plant height and grain weight. We identified two major haplotypes of TaB3-2A1 and found that TaB3-2A1-Hap1 conferred lower seed protein content, but higher starch content, plant height and grain weight than TaB3-2A1-Hap2 and was subjected to positive selection in a panel of elite wheat cultivars. These findings provide a high-efficiency tool to detect TFs binding to targeted promoters, considerable gene resources for dissecting regulatory mechanisms underlying Glu-1 expression, and a useful gene for wheat improvement.
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