ArticleViruses2023
Establishment and Application of a Triplex Real-Time RT-PCR Assay for Differentiation of PEDV, PoRV, and PDCoV.
Article in Viruses, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
25 citing papers in PubMed, 27 citations in OpenAlex.
- Development and clinical validation of a multiplex visual POCT platform based on RT-RAA-CRISPR/Cas12a for porcine diarrheal viruses.Synthetic and systems biotechnology · 2026Article
- Spatiotemporal distribution and molecular characteristics of porcine diarrhea-associated viruses detected in Chinese pigs.Virulence · 2026Article
- A crRNA/Cas12a complex-driven rapid and visual detection method for four porcine diarrhea viruses.BMC veterinary research · 2026Article
- Epidemiological characteristics of co-infection between porcine epidemic diarrhea virus (PEDV) and other pathogens: a meta-analysis and systematic review.Porcine health management · 2026Article
- Generation of G9 genotype porcine rotavirus using reverse genetics system and its application for antiviral screen and vaccine development.Virologica Sinica · 2026Article
- Swine Enteric Coronaviruses: An Updated Overview of Epidemiology, Diagnosis, Prevention, and Control.Animals : an open access journal from MDPI · 2026Review
- Establishment of a droplet digital PCR detection method for Vp4 gene of PoRV.Frontiers in veterinary science · 2026Article
- Establishment and Application of PDCoV Antibody Indirect ELISA Detection Method Based on N Protein.Veterinary sciences · 2025Article
- Advancements in Modern Nucleic Acid-Based Multiplex Testing Methodologies for the Diagnosis of Swine Infectious Diseases.Veterinary sciences · 2025Review
- CRISPR/Cas12a-Based One-Tube RT-RAA Assay for PoRV Genotyping.International journal of molecular sciences · 2025Article
- The biomechanical phenomena observed in the cell invasion pathway of porcine epidemic diarrhea virus: a review.Archives of virology · 2025Review
- A novel double-antibody sandwich ELISA based on monoclonal antibodies against the viral spike protein detects porcine deltacoronavirus infection.Microbiology spectrum · 2025Article
- Advances in porcine epidemic diarrhea virus research: genome, epidemiology, vaccines, and detection methods.Discover nano · 2025Review
- Isolation, Genomic Characterization, and Immunogenicity Evaluation of a G9P[23] Porcine Rotavirus Strain.Veterinary sciences · 2025Article
- Genome Characterization of Mammalian Orthoreovirus and Porcine Epidemic Diarrhea Virus Isolated from the Same Fattening Pig.Animals : an open access journal from MDPI · 2025Article
- Establishment and application of a triplex real-time PCR assay for detection of porcine circoviruses.Frontiers in veterinary science · 2025Article
- Rapid, sensitive, and visual detection of porcine rotavirus with RPA-CRISPR/Cas13.Frontiers in microbiology · 2025Article
- Development and Application of a TaqMan-Based One-Step Quadruplex Reverse Transcription Real-Time PCR (RT-qPCR) for Differential Detection of Four Porcine Diarrhea Viruses.Transboundary and emerging diseases · 2025Article
- Development and Clinical Application of a Molecular Assay for Four Common Porcine Enteroviruses.Veterinary sciences · 2024Article
- Lipidomics reveals the significance and mechanism of the cellular ceramide metabolism for rotavirus replication.Journal of virology · 2024Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Porcine viral diarrhea is very common in clinical practice and has caused huge losses to the pig industry. Porcine epidemic diarrhea virus (PEDV), porcine rotavirus (PoRV), and porcine deltacoronavirus (PDCoV) are important pathogens of porcine viral diarrhea. Co-infection situations among these three viruses in clinics are common, which increases the difficulty of differential diagnosis. Currently, polymerase chain reaction (PCR) is commonly used to detect pathogens. TaqMan real-time PCR is more sensitive than conventional PCR and has better specificity and accuracy. In this study, a triplex real-time RT-PCR assay based on TaqMan probes was developed for differential detection of PEDV, PoRV, and PDCoV. The triplex real-time RT-PCR assay developed in this study could not detect unrelated pathogens and showed satisfactory specificity, sensitivity, repeatability, and reproducibility with a limit of detection (LOD) of 6.0 × 10
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.