Evidence map›Paper›PMID 37370573›Full record

ArticleBioengineering (Basel, Switzerland)2023

Comparing Performance of Spectral Image Analysis Approaches for Detection of Cellular Signals in Time-Lapse Hyperspectral Imaging Fluorescence Excitation-Scanning Microscopy.

Marina Parker, Naga S Annamdevula, Donald Pleshinger, Zara Ijaz, Josephine Jalkh, Raymond Penn, Deepak Deshpande, Thomas C Rich, Silas J Leavesley

Open access · goldAbstract read
In one paragraph

Article in Bioengineering (Basel, Switzerland), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
1.6field-weighted citation impact, top 17% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 7 citations in OpenAlex.

  1. Article
  2. Article
  3. Review
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 1 country.

Marina ParkerDepartment of Chemical and Biomolecular Engineering, University of South Alabama, 150 Student Services Dr., Mobile, AL 36688, USA.ORCID 0000-0002-9808-1202
Naga S AnnamdevulaDepartment of Pharmacology, University of South Alabama, 5851 USA Drive N., Mobile, AL 36688, USA.
Donald PleshingerDepartment of Pharmacology, University of South Alabama, 5851 USA Drive N., Mobile, AL 36688, USA.
Zara IjazCollege of Medicine, University of South Alabama, 5851 USA Drive N., Mobile, AL 36688, USA.
Josephine JalkhCollege of Medicine, University of South Alabama, 5851 USA Drive N., Mobile, AL 36688, USA.
Raymond PennCollege of Medicine, Thomas Jefferson University, Philadelphia, PA 19107, USA.ORCID 0000-0001-8452-5880
Deepak DeshpandeCollege of Medicine, Thomas Jefferson University, Philadelphia, PA 19107, USA.ORCID 0000-0002-6187-5332
Thomas C RichDepartment of Pharmacology, University of South Alabama, 5851 USA Drive N., Mobile, AL 36688, USA.
Silas J LeavesleyDepartment of Chemical and Biomolecular Engineering, University of South Alabama, 150 Student Services Dr., Mobile, AL 36688, USA.ORCID 0000-0002-2684-9534
University of South Alabama · USThomas Jefferson University · US

Funding

T-type Calcium Channels and von Willebrand Factor ReleaseP01HL066299 · NHLBI · UNIVERSITY OF SOUTH ALABAMA · PI LEAVESLEY, SILAS JOSIAH · 2001 to 2022
$34.6M
G Protein-Coupled Receptor Signaling in Airway Smooth MuR01HL058506 · NHLBI · WAKE FOREST UNIVERSITY HEALTH SCIENCES · PI RAYMOND B. PENN · 2001 to 2026
$9.3M
Compartmentalized signaling and crosstalk in airway myocytesR01HL169522 · NHLBI · UNIVERSITY OF SOUTH ALABAMA · PI RENNOLDS S OSTROM, RAYMOND B. PENN · 2023 to 2026
$2.2M
Functional Diversity of Compartmentalized Calcium Signaling in Airway Smooth MuscleR01HL137030 · NHLBI · THOMAS JEFFERSON UNIVERSITY · PI DESHPANDE, DEEPAK A · 2018 to 2021
$2.2M
NHLBI NIH HHS P01 HL066299NHLBI NIH HHS R01 HL058506NHLBI NIH HHS R01 HL137030NHLBI NIH HHS R01 HL169522NIH HHS P01HL066299NIH HHS R01HL137030NIH HHS R01HL169522NIH HHS R01HL58506
6 · The paper itself

Abstract

Hyperspectral imaging (HSI) technology has been applied in a range of fields for target detection and mixture analysis. While HSI was originally developed for remote sensing applications, modern uses include agriculture, historical document authentication, and medicine. HSI has also shown great utility in fluorescence microscopy. However, traditional fluorescence microscopy HSI systems have suffered from limited signal strength due to the need to filter or disperse the emitted light across many spectral bands. We have previously demonstrated that sampling the fluorescence excitation spectrum may provide an alternative approach with improved signal strength. Here, we report on the use of excitation-scanning HSI for dynamic cell signaling studies-in this case, the study of the second messenger Ca

Indexed as

cellular autofluorescencefluorescence microscopyhyperspectral imagingtheoretical sensitivity

Identifiers

PMID37370573
PMCPMC10295298
OpenAlexW4378175239

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.