Evidence map›Paper›PMID 37370156›Full record

ArticleJournal of ovarian research2023

Amniotic fluid-derived exosomes attenuated fibrotic changes in POI rats through modulation of the TGF-β/Smads signaling pathway.

Nahideh Nazdikbin Yamchi, Shahin Ahmadian, Halimeh Mobarak, Farhad Amjadi, Rahim Beheshti, Amin Tamadon, Reza Rahbarghazi, Mahdi Mahdipour

Erratum issuedOpen access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of ovarian research, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 31 papers, 3 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
31citing papers in PubMed, 3 pooled it
5.7field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

31 citing papers in PubMed, 3 syntheses or guidelines pooled it, 37 citations in OpenAlex.

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4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 2 countries.

Nahideh Nazdikbin YamchiStem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
Shahin AhmadianStem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
Halimeh MobarakStem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
Farhad AmjadiStem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
Rahim BeheshtiFaculty of Veterinary Medicine, Shabestar Islamic Azad University, Shabestar, Iran.
Amin TamadonPerciaVista R&D Co., Shiraz, Iran.
Reza RahbarghaziDrug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
Mahdi MahdipourStem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran. mahdi.mahdipour@gmail.com.
Tabriz University of Medical Sciences · IRIslamic Azad University Shabestar · IRWest Kazakhstan Marat Ospanov State Medical University · KZ

Funding

Tabriz University of Medical Sciences IR.TBZMED.VCR.REC.1398.060
6 · The paper itself

Abstract

In the current study, we investigated the regenerative effects of amniotic fluid exosomes (AF-Exos) in a rat model for premature ovarian insufficiency (POI). POI is a condition characterized by a decrease in ovarian function that can lead to infertility. We induced POI by administering cyclophosphamide (CTX) for 15 consecutive days, and then transplanted AF-Exos directly into both ovarian tissues. Four weeks later, we measured the serum levels of follicle-stimulating hormone (FSH), luteinizing hormone (LH), and estradiol (E2), and performed histopathological evaluations using H & E and Masson's trichrome staining. We also monitored the expression of genes related to the TGF-β signaling pathway using real-time PCR and examined the fertility rate of POI rats after AF-Exos therapy. Histological analysis showed an increase in atretic follicles and a decrease in healthy follicle count after POI induction. Four weeks post-AF-Exos intervention, the healthy follicle count increased (p < 0.01) while the atretic follicle count decreased (p < 0.001). In parallel, the deposition of collagen fibers also decreased following AF-Exos transplantation. The concentrations of FSH and LH hormones in sera remained unchanged after injection of AF-Exos, while E2 levels increased (p < 0.05). The expression of Smad-4 (p < 0.01) and Smad-6 (p < 0.05) was upregulated in POI rats that received AF-Exos, while Smad-2, TGF-β1, TNF-α, and IL-10 remained statistically unchanged. Our records showed a notable increase in litter number after AF-Exos compared to the non-treated POI rats. These results suggest that AF-Exos transplantation has the potential to restore ovarian function through the TGF-β/Smads signaling pathway in POI rats.

Indexed as

ExosomesMenopause, PrematurePrimary Ovarian InsufficiencyAmniotic FluidAnimalsFemaleFollicle Stimulating HormoneRatsSignal TransductionTransforming Growth Factor betaTransforming Growth Factor beta1Follicle Stimulating HormoneTgfb1 protein, ratTransforming Growth Factor betaTransforming Growth Factor beta1Amniotic derived exosomeFertility restorationOvarian tissue regenerationPremature ovarian insufficiency

Identifiers

PMID37370156
PMCPMC10294370
OpenAlexW4382278765

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.