Evidence map›Paper›PMID 37369026›Full record

ArticleBioinformatics (Oxford, England)2023

Plassembler: an automated bacterial plasmid assembly tool.

George Bouras, Anna E Sheppard, Vijini Mallawaarachchi, Sarah Vreugde

Abstract read
In one paragraph

Article in Bioinformatics (Oxford, England), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 65 papers.

0numbers the graph read from it
0cells of the map it votes in
65citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

65 citing papers in PubMed.

  1. Article
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  4. Complete genome sequence of multidrug-resistantMicrobiology resource announcements · 2026
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  7. Complete genome sequences ofMicrobiology resource announcements · 2026
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  8. Complete genome sequence ofMicrobiology resource announcements · 2026
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  9. Article
  10. Draft genome assemblies of doxycycline-resistant derivatives ofMicrobiology resource announcements · 2026
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  11. Review
  12. Complete genome sequence of aMicrobiology resource announcements · 2026
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  13. Complete genome sequence data forMicrobiology resource announcements · 2026
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  14. ASM case reports · 2026
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  15. Article
  16. Article
  17. Genetic Characterization of Carbapenem-ResistantAntibiotics (Basel, Switzerland) · 2026
    Article
  18. Whole-genome sequences of 20Microbiology resource announcements · 2026
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  19. Article
  20. Carbapenemase-producing hypervirulentJournal of the Association of Medical Microbiology and Infectious Disease Canada = Journal officiel de l'Association pour la microbiologie medicale et l'infectiologie Canada · 2026
    Article

5 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

George BourasAdelaide Medical School, Faculty of Health and Medical Sciences, The University of Adelaide, Adelaide, South Australia 5005, Australia.ORCID 0000-0002-5885-4186
Anna E SheppardSchool of Biological Sciences, The University of Adelaide, Adelaide, South Australia 5005, Australia.
Vijini MallawaarachchiFlinders Accelerator for Microbiome Exploration, College of Science and Engineering, Flinders University, Bedford Park, Adelaide, South Australia 5042, Australia.ORCID 0000-0002-2651-8719
Sarah VreugdeAdelaide Medical School, Faculty of Health and Medical Sciences, The University of Adelaide, Adelaide, South Australia 5005, Australia.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

summaryWith recent advances in sequencing technologies, it is now possible to obtain near-perfect complete bacterial chromosome assemblies cheaply and efficiently by combining a long-read-first assembly approach with short-read polishing. However, existing methods for assembling bacterial plasmids from long-read-first assemblies often misassemble or even miss bacterial plasmids entirely and accordingly require manual curation. Plassembler was developed to provide a tool that automatically assembles and outputs bacterial plasmids using a hybrid assembly approach. It achieves increased accuracy and computational efficiency compared to the existing gold standard tool Unicycler by removing chromosomal reads from the input read sets using a mapping approach. AVAILABILITY AND IMPLEMENTATION: Plassembler is implemented in Python and is installable as a bioconda package using 'conda install -c bioconda plassembler'. The source code is available on GitHub at https://github.com/gbouras13/plassembler. The full benchmarking pipeline can be found at https://github.com/gbouras13/plassembler_simulation_benchmarking, while the benchmarking input FASTQ and output files can be found at https://doi.org/10.5281/zenodo.7996690.

Indexed as

High-Throughput Nucleotide SequencingSoftwarePlasmidsSequence Analysis, DNA

Identifiers

PMID37369026
PMCPMC10326302

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.