ArticlePLoS biology2023
A biologist's guide to planning and performing quantitative bioimaging experiments.
Article in PLoS biology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
26 citing papers in PubMed, 28 citations in OpenAlex.
- Spatially Controlled Capture and Site-Resolved Analysis of Single Extracellular Vesicles.Small (Weinheim an der Bergstrasse, Germany) · 2026Article
- Practical statistics for bioimage analysis - a guide to experimental design and data interpretation.Journal of cell science · 2026Article
- Training module and assessment strategy for light microscopy skills training in a research environment.Journal of microbiology & biology education · 2026Article
- JuNkIE-CLImax: exploring multidimensional images in notebooks and the terminal.microPublication biology · 2026Article
- There and back again: a cell biologist's journey from organelles to molecules.Biological chemistry · 2025Review
- Visualizing intraorganellar ultrastructures, dynamics, and interactions with open-access background-free Lock-in-SIM.Nature communications · 2025Article
- Image scanning microscopy based on multifocal metalens for sub-diffraction-limited imaging of brain organoids.Light, science & applications · 2025Article
- A checklist for designing and improving the visualization of scientific data.Nature cell biology · 2025Article
- F-BIAS: Towards a distributed national core facility for bioimage analysis.PLoS computational biology · 2025Article
- Bridging imaging-based in vitro methods from biomedical research to regulatory toxicology.Archives of toxicology · 2025Review
- Enhancing Research Through Image Analysis Workshops: Experiences and Best Practices.Microscopy research and technique · 2025Article
- Practical considerations for data exploration in quantitative cell biology.Journal of cell science · 2025Article
- Microscopy methods for the in vivo study of nanoscale nuclear organization.Biochemical Society transactions · 2025Review
- Trail-blazing and keeping pace: building, retaining and expanding image analysis expertise.Frontiers in bioinformatics · 2025Article
- The importance of democratized resources in early-career training for bioimage analysts and bioimaging scientists.Frontiers in bioinformatics · 2025Article
- Article
- Bridging imaging users to imaging analysis - A community survey.Journal of microscopy · 2024Article
- The crucial role of bioimage analysts in scientific research and publication.Journal of cell science · 2024Article
- Creating and troubleshooting microscopy analysis workflows: Common challenges and common solutions.Journal of microscopy · 2024Article
- Creating and troubleshooting microscopy analysis workflows: common challenges and common solutions.ArXiv · 2024Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
16 authors at 10 institutions in 4 countries.
Funding
Abstract
Technological advancements in biology and microscopy have empowered a transition from bioimaging as an observational method to a quantitative one. However, as biologists are adopting quantitative bioimaging and these experiments become more complex, researchers need additional expertise to carry out this work in a rigorous and reproducible manner. This Essay provides a navigational guide for experimental biologists to aid understanding of quantitative bioimaging from sample preparation through to image acquisition, image analysis, and data interpretation. We discuss the interconnectedness of these steps, and for each, we provide general recommendations, key questions to consider, and links to high-quality open-access resources for further learning. This synthesis of information will empower biologists to plan and execute rigorous quantitative bioimaging experiments efficiently.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.