Evidence map›Paper›PMID 37368744›Full record

ArticleTropical medicine and infectious disease2023

Simultaneous Detection of Influenza A/B, Respiratory Syncytial Virus, and SARS-CoV-2 in Nasopharyngeal Swabs by One-Tube Multiplex Reverse Transcription Polymerase Chain Reaction.

Bader S Alotaibi, Bilal Ahmad Tantry, Altaf Bandy, Reyaz Ahmad, Syed Quibtiya Khursheed, Arshid Ahmad, Mohammed Ageeli Hakami, Naveed Nazir Shah

Open access · goldAbstract read
In one paragraph

Article in Tropical medicine and infectious disease, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
2.6field-weighted citation impact, top 10% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 12 citations in OpenAlex.

  1. Update on Influenza.The American journal of the medical sciences · 2026
    Review
  2. Article
  3. Article
  4. Article
  5. Article
  6. Article
  7. Article
  8. Editorial: High-impact respiratory RNA virus diseases.Frontiers in veterinary science · 2023
    Article
  9. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 2 countries.

Bader S AlotaibiDepartment of Clinical Laboratory Science, College of Applied Medical Sciences, Shaqra University, Alquwayiyah 19257, Saudi Arabia.ORCID 0000-0001-6878-257X
Bilal Ahmad TantryDepartment of Microbiology, Government Medical College, Srinagar 190010, India.ORCID 0000-0002-3819-7158
Altaf BandyDepartment of Community Medicine, College of Medicine, Shaqra University, Shaqra 15273, Saudi Arabia.
Reyaz AhmadDepartment of Microbiology, Government Medical College, Srinagar 190010, India.
Syed Quibtiya KhursheedDepartment of Surgery, SKIMS Medical College, Srinagar 190018, India.
Arshid AhmadDepartment of Pulmonary Medicine, Government Medical College, Srinagar 190001, India.
Mohammed Ageeli HakamiDepartment of Clinical Laboratory Science, College of Applied Medical Sciences, Shaqra University, Alquwayiyah 19257, Saudi Arabia.ORCID 0000-0002-1747-1277
Naveed Nazir ShahDepartment of Pulmonary Medicine, Government Medical College, Srinagar 190001, India.ORCID 0000-0002-9486-6715
Government Medical College · INShaqra University · SASher-i-Kashmir Institute of Medical Sciences · IN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The treatment and outcome of respiratory virus infections differ. SARS-CoV-2, as well as other respiratory viruses such as influenza virus (A and B) and respiratory syncytial virus (RSV), require simultaneous, cost-effective, and rapid differential detection. We used a gold standard five-target single-step RT-PCR to detect influenza viruses, RSV, and SARS-CoV-2, and this method can be extended to detect influenza virus subtypes. As a result, this five-target single-step RT-PCR method is ideal for differentiating respiratory viruses. The 5' nuclease activity of Taq DNA polymerase is used in the real-time reverse transcription PCR assay. The Taq man fast viral 1-step enzyme is a 4× Master mix and five-target primer probe mix that detects influenza A, influenza B, SARS-CoV-2 ORF1ab, respiratory syncytial viruses A/B and actin. When compared with TaqMan

Indexed as

5-PLEX assayinfluenza Ainfluenza Breal-time reverse transcriptionRSVSARS-CoV-2

Identifiers

PMID37368744
PMCPMC10305207
OpenAlexW4381251371

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.