ArticleJournal of fungi (Basel, Switzerland)2023
Single-Point Nail Sampling to Diagnose Onychomycosis Caused by Non-Dermatophyte Molds: Utility of Polymerase Chain Reaction (PCR) and Histopathology.
Article in Journal of fungi (Basel, Switzerland), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed.
- Specific Primers and Nested PCR FindJournal of fungi (Basel, Switzerland) · 2025Article
- Analysis of Causative Factors and Potential Predictors of Onychomycosis: A Retrospective Single-Center Study in Poland.Journal of fungi (Basel, Switzerland) · 2025Article
- Monocentric evaluation of the Novaplex dermatophyte multiplex qPCR assay in the diagnosis of dermatophytoses.Journal of clinical microbiology · 2024Article
- Epidemiology of Onychomycosis in the United States Characterized Using Molecular Methods, 2015-2024.Journal of fungi (Basel, Switzerland) · 2024Article
- Transungual Penetration and Antifungal Activity of Prescription and Over-the-Counter Topical Antifungals: Ex Vivo Comparison.Dermatology and therapy · 2024Article
- New Diagnostic Strategy for Onychomycosis: First-Line Utilization of DermaGeniusJournal of fungi (Basel, Switzerland) · 2024Article
- COSkin appendage disorders · 2024Article
- Clinical Diagnosis and Laboratory Testing of Abnormal Appearing Toenails: A Retrospective Assessment of Confirmatory Testing for Onychomycosis in the United States, 2022-2023.Journal of fungi (Basel, Switzerland) · 2024Article
- Detection of Dermatophytes in Subclinically Involved Nails by Polymerase Chain Reaction in Patients with Skin Dermatophytosis: A Prospective Observational Study.Indian journal of dermatologyArticle
- Onychomycosis in the US Pediatric Population-An Emphasis on Fusarium Onychomycosis.Pediatric dermatologyArticle
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5 authors.
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Abstract
The three most commonly used methods for diagnosing non-dermatophyte mold (NDM) onychomycosis are culture, polymerase chain reaction (PCR), and histopathology. Toenail samples from 512 patients (1 sample/patient) with suspected onychomycosis were examined using all three diagnostic tests. A statistically significant association was found between PCR and histopathology results, as well as between fungal culture and histopathology results. All PCR-positive and culture-positive dermatophyte samples were confirmed by histopathology. However, 15/116 (12.9%) of culture-positive NDM samples had negative histopathology results, while all PCR-positive NDM samples were confirmed by histopathology. The overall rate of dermatophyte detection was higher using PCR compared to culture (38.9% vs. 11.7%); the lower rate of NDM detection by PCR (11.7% vs. 38.9%) could be attributed to the restriction of the assay design to seven pre-selected targets. When repeat sampling in the clinic is not possible, a combination of NDM detection by PCR and positive histopathology of hyphae may be a proxy for NDM infection, particularly where the NDM occurs without a concomitant dermatophyte. There was a high degree of correlation between negative PCR and negative histopathology. A negative PCR result with negative histopathology findings may be a reliable proxy for the diagnosis of non-fungal dystrophy.
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