Evidence map›Paper›PMID 37367469›Full record

ArticleJournal of imaging2023

Live Cell Light Sheet Imaging with Low- and High-Spatial-Coherence Detection Approaches Reveals Spatiotemporal Aspects of Neuronal Signaling.

Mariana Potcoava, Donatella Contini, Zachary Zurawski, Spencer Huynh, Christopher Mann, Jonathan Art, Simon Alford

Abstract read
In one paragraph

Article in Journal of imaging, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Mariana PotcoavaDepartment of Anatomy and Cell Biology, University of Illinois at Chicago, 808 South Wood Street, Rm 578 MC 512, Chicago, IL 60612, USA.
Donatella ContiniDepartment of Anatomy and Cell Biology, University of Illinois at Chicago, 808 South Wood Street, Rm 578 MC 512, Chicago, IL 60612, USA.
Zachary ZurawskiDepartment of Anatomy and Cell Biology, University of Illinois at Chicago, 808 South Wood Street, Rm 578 MC 512, Chicago, IL 60612, USA.
Spencer HuynhDepartment of Anatomy and Cell Biology, University of Illinois at Chicago, 808 South Wood Street, Rm 578 MC 512, Chicago, IL 60612, USA.
Christopher MannDepartment of Applied Physics and Materials Science, Northern Arizona University, Flagstaff, AZ 86011, USA.
Jonathan ArtDepartment of Anatomy and Cell Biology, University of Illinois at Chicago, 808 South Wood Street, Rm 578 MC 512, Chicago, IL 60612, USA.
Simon AlfordDepartment of Anatomy and Cell Biology, University of Illinois at Chicago, 808 South Wood Street, Rm 578 MC 512, Chicago, IL 60612, USA.

Funding

Synaptic Physiology of the Vestibular PeripheryR01DC019953 · NIDCD · UNIVERSITY OF ILLINOIS AT CHICAGO · PI JONATHAN JAMES ART, LARRY F HOFFMAN · 2021 to 2026
$3.3M
Regulation of exocytosis by direct Gbg blockade of fusionR01NS111749 · NINDS · VANDERBILT UNIVERSITY · PI ALFORD, SIMON T, HAMM, HEIDI E · 2019 to 2023
$2.0M
The biophysical roles of hair cell and calyx afferent ion channels in synaptic transmission between vestibular receptors and afferent nerves in mammals.R21DC017577 · NIDCD · UNIVERSITY OF ILLINOIS AT CHICAGO · PI CONTINI, DONATELLA · 2019 to 2021
$480k
Optical and biophysical characterization of the vestibular peripheryR21DC017292 · NIDCD · UNIVERSITY OF ILLINOIS AT CHICAGO · PI ART, JONATHAN JAMES · 2019 to 2020
$440k
NIDCD NIH HHS R01 DC019953NIDCD NIH HHS R21 DC017292NIDCD NIH HHS R21 DC017577NIH HHS RO1 NS111749, R21 DC017292, R21 DC017577NINDS NIH HHS R01 NS111749
6 · The paper itself

Abstract

Light sheet microscopy in live cells requires minimal excitation intensity and resolves three-dimensional (3D) information rapidly. Lattice light sheet microscopy (LLSM) works similarly but uses a lattice configuration of Bessel beams to generate a flatter, diffraction-limited z-axis sheet suitable for investigating subcellular compartments, with better tissue penetration. We developed a LLSM method for investigating cellular properties of tissue in situ. Neural structures provide an important target. Neurons are complex 3D structures, and signaling between cells and subcellular structures requires high resolution imaging. We developed an LLSM configuration based on the Janelia Research Campus design or in situ recording that allows simultaneous electrophysiological recording. We give examples of using LLSM to assess synaptic function in situ. In presynapses, evoked Ca

Indexed as

electrophysiology and simultaneous imagingfluorescence microscopyincoherent lattice light sheetin situ recordinglattice light sheet holographysynaptic functiontomographic imaging

Identifiers

PMID37367469
PMCPMC10299414

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.