ReviewCurrent issues in molecular biology2023
Sperm Cryopreservation Today: Approaches, Efficiency, and Pitfalls.
Review in Current issues in molecular biology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 59 papers, 2 of them syntheses that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
59 citing papers in PubMed, 2 syntheses or guidelines pooled it.
- ESHRE recommendations on Good Practice in the IVF laboratory†.Human reproduction (Oxford, England) · 2026Guideline
- "Management of andrological disorders from childhood and adolescence to transition age: guidelines from the Italian Society of Andrology and Sexual Medicine (SIAMS) in collaboration with the Italian Society for Pediatric Endocrinology and Diabetology (SIEDP)-Part-1".Journal of endocrinological investigation · 2025Guideline
- Analysis of the Osmolarity of the Liquid Fraction of Squirrel Monkey's Semen (Saimiri collinsi Osgood, 1916) in Fattening and Non-Fattening Conditions.Journal of medical primatology · 2026Article
- Fresh versus frozen-thawed testicular sperm for intracytoplasmic sperm injection in men with non-obstructive azoospermia: a systematic review and meta-analysis.Journal of assisted reproduction and genetics · 2026Review
- Evaluation of sperm kinematics, physical integrity and malondialdehyde levels as indicators of sperm freezability in Pradu Hang Dam chicken.Veterinary research communications · 2026Article
- Comparative Evaluation of TriladylVeterinary sciences · 2026Article
- Optimize human sperm cryopreservation: influence of vial sealing methods and automated freezing protocols on preserved sperm quality.Translational andrology and urology · 2026Article
- Coenzyme Q10 supplementation enhances HSP70 gene expression and preserves mitochondrial function in cryopreserved Peranakan Ettawa goat spermatozoa.Veterinary world · 2026Article
- Impact of Environmental Conditions During IVF Laboratory Handling on Sperm Quality.International journal of molecular sciences · 2026Article
- Article
- Optimization of Semen Extender for Mongolian Sheep by Integrating Metabolomics and Sperm Quality Analysis at 17 °C with Antioxidant Supplementation.Animals : an open access journal from MDPI · 2026Article
- Post-thaw quality of donkey (Equus asinus) spermatozoa exposed to different concentrations of curcumin with respect to oxidative status and nitric oxide.Veterinary research communications · 2026Review
- Chemotherapy-Induced Infertility: Mechanisms, Risk Factors, and Fertility Preservation Strategies in Cancer Patients.JBRA assisted reproduction · 2026Review
- Comparison of three commercial sperm freezing media with regard to the influence on sperm motility, vitality and DNA fragmentation.Translational andrology and urology · 2026Article
- Cryopreservation of Aseel rooster sperm using different glycerol-iodixanol combinations and evaluation of post-thaw quality.BMC veterinary research · 2026Article
- Physalis polysaccharide-sericin nanocarriers for controlled alpha-lipoic acid delivery and improved human sperm cryopreservation.Scientific reports · 2026Article
- Oxidative Stress and Male Reproductive Health-First Edition.Antioxidants (Basel, Switzerland) · 2026Article
- Protective effects of coenzyme Q10-supplemented cryopreservation medium on oxidative stress in frozen canine sperm.Veterinary research communications · 2026Article
- Current status and advances in bovine semen production technologies for artificial insemination in Japan.The Journal of reproduction and development · 2026Review
- The Effects of Graphene Oxide Nanoparticles on the Cryopreservation of Angora Buck Sperm.Molecules (Basel, Switzerland) · 2026Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
Abstract
The cryopreservation of human spermatozoa has been an option for patients undergoing chemo or radiotherapies since the late 1950s. Presently, there are different techniques for the cryopreservation of spermatozoa. The most commonly used techniques are programmable slow freezing and freezing on liquid nitrogen vapors, while the use of vitrification is still not accepted as clinically relevant. Although there have been many improvements, the ideal technique for achieving better post-thaw sperm quality continues to be a mystery. A major obstacle during cryopreservation is the formation of intracellular ice crystals. Cryodamage generated by cryopreservation causes structural and molecular alterations in spermatozoa. Injuries can happen because of oxidative stress, temperature stress, and osmotic stress, which then result in changes in the plasma membrane fluidity, motility, viability, and DNA integrity of the spermatozoa. To prevent cryodamage as much as possible, cryoprotectants are added, and in some clinical trial cases, even antioxidants that may improve post-thaw sperm quality are added. This review discusses cryopreservation techniques, cryodamage on molecular and structural levels, and cryoprotectants. It provides a comparison of cryopreservation techniques and describes recent advances in those techniques.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.