Evidence map›Paper›PMID 37366163›Full record

ArticleThe American journal of sports medicine2023

Acute Bone Loss and Infrapatellar Fat Pad Fibrosis in the Knee After an In Vivo ACL Injury in Adolescent Mice.

Taeyong Ahn, Benjamin E Loflin, Nicholas B Nguyen, Ciena K Miller, Kaitlyn A Colglazier, Edward M Wojtys, Stephen H Schlecht

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The American journal of sports medicine, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed, 1 pooled it
1.3field-weighted citation impact, top 20% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 1 synthesis or guideline pooled it, 4 citations in OpenAlex.

  1. Pooled it
  2. Article
  3. Article
  4. Article
  5. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 3 institutions in 1 country.

Taeyong AhnDepartment of Orthopaedic Surgery, Indiana University School of Medicine, Indianapolis, Indiana, USA.
Benjamin E LoflinDepartment of Orthopaedic Surgery, Indiana University School of Medicine, Indianapolis, Indiana, USA.
Nicholas B NguyenIndiana University School of Medicine, Evansville, Indiana, USA.
Ciena K MillerDepartment of Orthopaedic Surgery, Indiana University School of Medicine, Indianapolis, Indiana, USA.
Kaitlyn A ColglazierDepartment of Biomedical Engineering, Indiana University Purdue University-Indianapolis, Indianapolis, Indiana, USA.
Edward M WojtysDepartment of Orthopaedic Surgery, University of Michigan, Ann Arbor, Michigan, USA.
Stephen H SchlechtDepartment of Orthopaedic Surgery, Indiana University School of Medicine, Indianapolis, Indiana, USA.ORCID 0000-0002-6857-927X
Indiana University School of MedicineUniversity of Indianapolis · USUniversity of Michigan · US

Funding

Natural Variation in the Growth and Development of the ACL ComplexR00AR070903 · NIAMS · INDIANA UNIVERSITY INDIANAPOLIS · PI SCHLECHT, STEPHEN · 2019 to 2021
$739k
NIAMS NIH HHS L40 AR072482NIAMS NIH HHS R00 AR070903
6 · The paper itself

Abstract

backgroundYoung patients are 6 times more likely than adults to have a primary anterior cruciate ligament (ACL) graft failure. Biological factors (ie, tunnel osteolysis) may account for up to a third of these failures. Previous evaluations of patient ACL explants indicated significant bone loss within the entheseal regions. However, it remains unknown if the degree of bone loss within the ACL insertion regions, wherein ACL grafts are fixated, exceeds that of the femoral and tibial condylar bone. HYPOTHESIS: Bone loss in the mineralized matrices of the femoral and tibial ACL entheses is distinct from that clinically reported across the whole knee after injury. STUDY

designControlled laboratory study.

methodsWe developed a clinically relevant in vivo mouse ACL injury model to cross-sectionally track the morphological and physiological postinjury changes within the ACL, femoral and tibial entheses, synovial joint space, and load-bearing epiphyseal cortical and trabecular bone components of the knee joint. Right ACLs of 10-week-old C57BL/6J female mice (N = 75) were injured in vivo with the contralateral ACLs serving as controls. Mice were euthanized at 1, 3, 7, 14, or 28 days after injury (n = 12/cohort). Downstream analyses included volumetric cortical and trabecular bone analyses and histopathologic assessments of the knee joint after injury. Gait analyses across all time points were also performed (n = 15 mice).

resultsThe majority of the ACL injuries in mice were partial tears. The femoral and tibial cortical bone volumes were 39% and 32% lower, respectively, at 28 days after injury than those of the uninjured contralateral knees (

conclusionBone loss was acute and persisted for 4 weeks after injury in mice. However, the authors' hypothesis was not confirmed, as bone quality was not significantly lower in the entheses compared with the condylar bone regions after injury. With relatively normal hindlimb loading but a significant physiological response after injury, bone loss in this model may be driven by inflammation. CLINICAL RELEVANCE: There is persistent bone resorption and fibrotic tissue development after injury that is not resolved. Inflammatory and catabolic activity may have a significant role in the postinjury decline of bone quality in the knee.

Indexed as

Anterior Cruciate Ligament InjuriesAnimalsAnterior Cruciate LigamentFemaleFibrosisKnee JointMiceMice, Inbred C57BLACL injurybone lossinfrapatellar fat pad fibrosismouse modelsynovitis

Identifiers

PMID37366163
PMCPMC10529334
OpenAlexW4382181739

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.