Evidence map›Paper›PMID 37366074›Full record

ArticleJournal of animal science2023

Integrating sperm cell transcriptome and seminal plasma metabolome to analyze the molecular regulatory mechanism of sperm motility in Holstein stud bulls.

Wenlong Li, Siyuan Mi, Jinning Zhang, Xueqin Liu, Siqian Chen, Shuli Liu, Xia Feng, Yongjie Tang, Yanhua Li, Lin Liu and 3 more

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of animal science, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
2.5field-weighted citation impact, top 11% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 9 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Article
  5. Review
  6. Article
  7. Article
  8. Article
  9. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors at 3 institutions in 3 countries.

Wenlong LiKey Laboratory of Animal Genetics, Breeding and Reproduction, Ministry of Agriculture and Rural Affairs & National Engineering Laboratory for Animal Breeding, Department of Animal Breeding and Genetics, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China.
Siyuan MiKey Laboratory of Animal Genetics, Breeding and Reproduction, Ministry of Agriculture and Rural Affairs & National Engineering Laboratory for Animal Breeding, Department of Animal Breeding and Genetics, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China.
Jinning ZhangKey Laboratory of Animal Genetics, Breeding and Reproduction, Ministry of Agriculture and Rural Affairs & National Engineering Laboratory for Animal Breeding, Department of Animal Breeding and Genetics, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China.
Xueqin LiuKey Laboratory of Animal Genetics, Breeding and Reproduction, Ministry of Agriculture and Rural Affairs & National Engineering Laboratory for Animal Breeding, Department of Animal Breeding and Genetics, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China.
Siqian ChenKey Laboratory of Animal Genetics, Breeding and Reproduction, Ministry of Agriculture and Rural Affairs & National Engineering Laboratory for Animal Breeding, Department of Animal Breeding and Genetics, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China.
Shuli LiuKey Laboratory of Animal Genetics, Breeding and Reproduction, Ministry of Agriculture and Rural Affairs & National Engineering Laboratory for Animal Breeding, Department of Animal Breeding and Genetics, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China.
Xia FengKey Laboratory of Animal Genetics, Breeding and Reproduction, Ministry of Agriculture and Rural Affairs & National Engineering Laboratory for Animal Breeding, Department of Animal Breeding and Genetics, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China.
Yongjie TangKey Laboratory of Animal Genetics, Breeding and Reproduction, Ministry of Agriculture and Rural Affairs & National Engineering Laboratory for Animal Breeding, Department of Animal Breeding and Genetics, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China.
Yanhua LiBeijing Dairy Cattle Center, Qinghe'nanzhen Deshengmenwai Road, Beijing 100192, China.
Lin LiuBeijing Dairy Cattle Center, Qinghe'nanzhen Deshengmenwai Road, Beijing 100192, China.
Lingzhao FangMRC Human Genetics Unit, Institute of Genetics and Molecular Medicine, University of Edinburgh, Edinburgh EH4 2XU, UK.
Shengli ZhangKey Laboratory of Animal Genetics, Breeding and Reproduction, Ministry of Agriculture and Rural Affairs & National Engineering Laboratory for Animal Breeding, Department of Animal Breeding and Genetics, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China.
Ying YuKey Laboratory of Animal Genetics, Breeding and Reproduction, Ministry of Agriculture and Rural Affairs & National Engineering Laboratory for Animal Breeding, Department of Animal Breeding and Genetics, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China.
Ministry of Agriculture and Rural Affairs · CNChina Agricultural University · CNAarhus University · DK

Funding

China Agriculture Research SystemEarmarked Fund for China Agriculture Research System CARS-36MARAMOFNational Key R&D Program of China 2021YFD1200903National Natural Science Foundation of China 32072718Seed Fund
6 · The paper itself

Abstract

Considering that artificial insemination is the most widely used assisted reproductive technique in the dairy industry, the semen quality of bulls is very important for selecting excellent stud bulls. Sperm motility is one of the important traits of semen quality, and related genes may be regulated by environmental factors. Seminal plasma can affect sperm cell transcriptome and further affect sperm motility through exosome or other processes. However, the molecular regulation mechanism of bull sperm motility has not been studied by combining the sperm cell transcriptome with seminal plasma metabolome. The number of motile sperm per ejaculate (NMSPE) is an integrated indicator for assessing sperm motility in stud bulls. In the present study, we selected 7 bulls with higher NMSPE (5,698.55 million +/- 945.40 million) as group H and 7 bulls with lower NMSPE (2,279.76 million +/- 1,305.69 million) as group L from 53 Holstein stud bulls. The differentially expressed genes (DEGs) in sperm cells were evaluated between the two groups (H vs. L). We conducted gene co-expression network analysis (WGCNA) on H and L groups of bulls, as well as two monozygotic twin Holstein bulls with different NMSPE values, to screen candidate genes for NMSPE. The regulatory effect of seminal plasma metabolome on the candidate genes of NMSPE was also investigated. A total of 1,099 DEGs were identified in the sperm cells of H and L groups. These DEGs were primarily concentrated in energy metabolism and sperm cell transcription. The significantly enriched Kyoto encyclopedia of genes and genomes (KEGG) pathways of the 57 differential metabolites were the aminoacyl-tRNA biosynthesis pathway and vitamin B6 metabolism pathway. Our study discovered 14 genes as the potential candidate markers for sperm motility, including FBXO39. We observed a broad correlation between transcriptome of sperm cells and seminal plasma metabolome, such as three metabolites, namely, mesaconic acid, 2-coumaric acid, and 4-formylaminoantipyrine, might regulate FBXO39 expression through potential pathways. The genes related to seminal plasma metabolites expressed in sperm cells are not only located near the quantitative trait loci of reproductive traits, but also enriched in the genome-wide association study signal of sire conception rate. Collectively, this study was the first to investigate the interplays among transcriptome of sperm cells and seminal plasma metabolome from Holstein stud bulls with different sperm motility.

Indexed as

SemenSemen AnalysisAnimalsCattleGenome-Wide Association StudyMaleMetabolomeSpermatozoaSperm MotilityTranscriptomeChinese Holstein bullGWAS enrichmentseminal plasma metabolitessperm cell transcriptomesperm motility

Identifiers

PMID37366074
PMCPMC10355371
OpenAlexW4382182507

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.