Evidence map›Paper›PMID 37365470›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2023

Measurement of Salmonella enterica Internalization and Vacuole Lysis in Epithelial Cells.

Jessica A Klein, TuShun R Powers, Leigh A Knodler

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
1.2field-weighted citation impact, top 22% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 2 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Jessica A KleinPaul G. Allen School for Global Health, College of Veterinary Medicine, Washington State University, Pullman, WA, USA.
TuShun R PowersPaul G. Allen School for Global Health, College of Veterinary Medicine, Washington State University, Pullman, WA, USA.
Leigh A KnodlerPaul G. Allen School for Global Health, College of Veterinary Medicine, Washington State University, Pullman, WA, USA. leigh.knodler@med.uvm.edu.
Washington State University · US

Funding

Infectious Diseases and Microbial Immunology Training ProgramT32AI007025 · NIAID · WASHINGTON STATE UNIVERSITY · PI Thomas H KAWULA, M KARIUKI NJENGA · 1985 to 2026
$7.4M
6 · The paper itself

Abstract

Establishment of an intracellular niche within mammalian cells is key to the pathogenesis of the gastrointestinal bacterium, Salmonella enterica serovar Typhimurium (S. Typhimurium). Here we will describe how to study the internalization of S. Typhimurium into human epithelial cells using the gentamicin protection assay. The assay takes advantage of the relatively poor penetration of gentamicin into mammalian cells; internalized bacteria are effectively protected from its antibacterial actions. A second assay, the chloroquine (CHQ) resistance assay, can be used to determine the proportion of internalized bacteria that have lysed or damaged their Salmonella-containing vacuole and are therefore residing within the cytosol. Its application to the quantification of cytosolic S. Typhimurium in epithelial cells will also be presented. Together, these protocols provide an inexpensive, rapid, and sensitive quantitative measure of bacterial internalization and vacuole lysis by S. Typhimurium.

Indexed as

Salmonella entericaAnimalsBacterial ProteinsEpithelial CellsGentamicinsHumansMammalsSalmonella typhimuriumVacuolesBacterial ProteinsGentamicinsChloroquine resistance assayColony-forming unitsEpithelial cellsGentamicin protection assaySalmonella-containing vacuoleSalmonella entericaType III secretion systemVacuole lysis

Identifiers

PMID37365470
OpenAlexW4382047176

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.