Evidence map›Paper›PMID 37365469›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2023

Quantitative Spatio-temporal Analysis of Phagosome Maturation in Live Cells.

Patricia Rosell Arévalo, Beren Aylan, Maximiliano G Gutierrez

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Patricia Rosell ArévaloHost-Pathogen Interactions in Tuberculosis Laboratory, The Francis Crick Institute, London, UK.
Beren AylanHost-Pathogen Interactions in Tuberculosis Laboratory, The Francis Crick Institute, London, UK.
Maximiliano G GutierrezHost-Pathogen Interactions in Tuberculosis Laboratory, The Francis Crick Institute, London, UK. max.g@crick.ac.uk.

Funding

Cancer Research UKMedical Research CouncilWellcome Trust
6 · The paper itself

Abstract

Phagocytosis and phagosome maturation are central processes to the development of the innate and adaptive immune response. Phagosome maturation is a continuous and dynamic process that occurs rapidly. In this chapter we describe fluorescence-based live cell imaging methods for the quantitative and temporal analysis of phagosome maturation of beads and M. tuberculosis as two phagocytic targets. We also describe simple protocols for monitoring phagosome maturation: the use of the acidotropic probe LysoTracker and analyzing the recruitment of EGFP-tagged host proteins by phagosomes.

Indexed as

Mycobacterium tuberculosisPhagocytosisPhagosomesSpatio-Temporal AnalysisLive cell imagingLysosomeMacrophageMycobacteriumPhagosome

Identifiers

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.