Evidence map›Paper›PMID 37365462›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2023

Super-Resolution Spinning-Disk Confocal Microscopy Using Optical Photon Reassignment (SoRa) to Visualize the Actin Cytoskeleton in Macrophages.

Freyja Verth, Gregory D Fairn

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
2.6field-weighted citation impact, top 10% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 2 citations in OpenAlex.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors at 1 institution in 1 country.

Freyja VerthDepartment of Pathology, Dalhousie University, Halifax, NS, Canada.
Gregory D FairnDepartment of Pathology, Dalhousie University, Halifax, NS, Canada. gfairn@dal.ca.
Dalhousie University · CA

Funding

CIHR PJT1655968
6 · The paper itself

Abstract

Macrophages are motile, morphologically plastic cells that undergo substantial cytoskeletal remodeling to facilitate their roles in innate and adaptive immunity. Macrophages are adept at producing a variety of specialized actin-driven structures and processes including the formation of podosomes and the ability to engulf particles through phagocytosis and sample large amounts of extracellular fluid via micropinocytosis. Here, we describe techniques for immunostaining proteins and transfecting macrophages with plasmids for use with either fixed or live cell imaging. Furthermore, we discuss the use of spinning-disk super-resolution using optical reassignment to generate sub-diffraction limited structures using this type of confocal microscope.

Indexed as

Actin CytoskeletonPodosomesActinsMacrophagesMicroscopy, ConfocalActinsActinConfocal microscopyMacrophagePodosomesSuper-resolutionVinculin

Identifiers

PMID37365462
OpenAlexW4382120097

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.