Evidence map›Paper›PMID 37363079›Full record

ArticleACS bio & med chem Au2023

Multiplex Protein Imaging through PACIFIC: Photoactive Immunofluorescence with Iterative Cleavage.

Fei Ji, Moises Hur, Sungwon Hur, Siwen Wang, Priyanka Sarkar, Shiqun Shao, Desiree Aispuro, Xu Cong, Yanhao Hu, Zhonghan Li and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in ACS bio & med chem Au, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Single-cell spatial proteomics.Histology and histopathology · 2025
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Fei JiDepartment of Chemistry, University of California, Riverside, Riverside, California 92521, United States.
Moises HurMartin Luther King Jr High School, Riverside, California 92508, United States.
Sungwon HurMartin Luther King Jr High School, Riverside, California 92508, United States.
Siwen WangDepartment of Chemistry, University of California, Riverside, Riverside, California 92521, United States.
Priyanka SarkarDepartment of Chemistry, University of California, Riverside, Riverside, California 92521, United States.
Shiqun ShaoDepartment of Chemistry, University of California, Riverside, Riverside, California 92521, United States.ORCID https://orcid.org/0000-0001-7029-3791
Desiree AispuroDepartment of Chemistry, University of California, Riverside, Riverside, California 92521, United States.
Xu CongDepartment of Chemistry, University of California, Riverside, Riverside, California 92521, United States.
Yanhao HuDiamond Bar High School, Diamond Bar, California 91765, United States.
Zhonghan LiDepartment of Chemistry, University of California, Riverside, Riverside, California 92521, United States.
Min XueDepartment of Chemistry, University of California, Riverside, Riverside, California 92521, United States.ORCID https://orcid.org/0000-0002-8136-6551

Funding

Research Training in Environmental ToxicologyT32ES018827 · NIEHS · UNIVERSITY OF CALIFORNIA RIVERSIDE · PI Yinsheng Wang · 2010 to 2026
$5.3M
Interrogation and interpretation of protein kinase signaling dynamics at single-cell resolutionR35GM138214 · NIGMS · UNIVERSITY OF CALIFORNIA RIVERSIDE · PI Min Xue · 2020 to 2026
$2.2M
NIEHS NIH HHS T32 ES018827NIGMS NIH HHS R35 GM138214
6 · The paper itself

Abstract

Multiplex protein imaging technologies enable deep phenotyping and provide rich spatial information about biological samples. Existing methods have shown great success but also harbored trade-offs between various pros and cons, underscoring the persisting necessity to expand the imaging toolkits. Here we present PACIFIC: photoactive immunofluorescence with iterative cleavage, a new modality of multiplex protein imaging methods. PACIFIC achieves iterative multiplexing by implementing photocleavable fluorophores for antibody labeling with one-step spin-column purification. PACIFIC requires no specialized instrument, no DNA encoding, or chemical treatments. We demonstrate that PACIFIC can resolve cellular heterogeneity in both formalin-fixed paraffin-embedded (FFPE) samples and fixed cells. To further highlight how PACIFIC assists discovery, we integrate PACIFIC with live-cell tracking and identify phosphor-p70S6K as a critical driver that governs U87 cell mobility. Considering the cost, flexibility, and compatibility, we foresee that PACIFIC can confer deep phenotyping capabilities to anyone with access to traditional immunofluorescence platforms.

Identifiers

PMID37363079
PMCPMC10288499

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.