ArticleComprehensive psychoneuroendocrinology2023
Inaccuracies in plasma oxytocin extraction and enzyme immunoassay techniques.
Article in Comprehensive psychoneuroendocrinology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers, 2 of them syntheses that pooled it.
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Who cites it
11 citing papers in PubMed, 2 syntheses or guidelines pooled it, 17 citations in OpenAlex.
- Music's context-dependent influence on oxytocin, social bonding, and emotion regulation: a systematic review.Frontiers in cognition · 2025Pooled it
- Systematic review: pain, cognition, and cardioprotection-unpacking oxytocin's contributions in a sport context.Frontiers in physiology · 2024Pooled it
- Quantification of appetite-regulating hormones in children with hypothalamic and common obesity.Journal of the Endocrine Society · 2026Article
- Salivary Oxytocin Levels in Children With and Without Autism: Group Similarities and Subgroup Variability.Journal of clinical medicine · 2025Article
- The Oxytocin System and Implications for Oxytocin Deficiency in Hypothalamic-Pituitary Disease.Endocrine reviews · 2025Review
- Association between oxytocin and S100B in community-dwelling older adults.PCN reports : psychiatry and clinical neurosciences · 2025Article
- Eating behavior patterns, metabolic parameters and circulating oxytocin levels in patients with obesity: an exploratory study.Eating and weight disorders : EWD · 2025Observational
- Trajectories of changes in oxytocin and vasopressin before, during, and after mother-infant interaction: a descriptive study of mothers and infants affected by postpartum depression.Frontiers in psychiatry · 2025Article
- Neurophysin I is an analytically robust surrogate biomarker for oxytocin.Psychoneuroendocrinology · 2024Article
- The psychophysiology of music-based interventions and the experience of pain.Frontiers in psychology · 2024Review
- Changes in salivary oxytocin in response to biologically-relevant events in farm animals: method optimization and usefulness as a biomarker.Frontiers in physiology · 2024Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Numerous studies have reported extensive associations between plasma oxytocin (OXT) concentrations and various human physiological and neurobehavioral processes. Measurement of OXT is fraught with difficulty due to its low molecular weight and plasma concentrations, with no consensus as to the optimal conditions for pre-analytical sample extraction, standards for immunoassay validation or the ideal protease inhibitors to prevent OXT degradation. Previous attempts at determining the efficacy of various purification techniques such as solid phase extraction (SPE) or ultrafiltration have only utilized human plasma samples, making it difficult to dissect out whether the effect of interference comes from the extraction process itself or cross-reactivity with other proteins. By testing these on pure OXT solutions, we demonstrate poor recovery efficacy and reliability of reversed phase SPE (maximum 58.1%) and ultrafiltration (<1%) techniques, and the potential for the former to introduce interference into enzyme immunoassay (EIA) measurements. The clonality of antibodies used in EIA kits also potentially contributes to the differences in the readings obtained, and we validate an EIA kit which did not require pre-analytical sample extraction with low cross-reactivity and high reliability (intraclass correlation coefficient 0.980 (95% CI 0.896-0.999). Biochemical techniques used for measuring plasma OXT concentrations must therefore be internally validated prior to translation into clinical studies.
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