Evidence map›Paper›PMID 37357983›Full record

ArticleAdvanced science (Weinheim, Baden-Wurttemberg, Germany)2023

Reduction of Intracellular Tension and Cell Adhesion Promotes Open Chromatin Structure and Enhances Cell Reprogramming.

Jennifer Soto, Yang Song, Yifan Wu, Binru Chen, Hyungju Park, Navied Akhtar, Peng-Yuan Wang, Tyler Hoffman, Chau Ly, Junren Sia and 7 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Advanced science (Weinheim, Baden-Wurttemberg, Germany), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.

0numbers the graph read from it
0cells of the map it votes in
13citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

13 citing papers in PubMed.

  1. Review
  2. Article
  3. Article
  4. Review
  5. Article
  6. Article
  7. Article
  8. Review
  9. Article
  10. Article
  11. A Hierarchical Mechanotransduction System: From Macro to Micro.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024
    Review
  12. Article
  13. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

17 authors.

Jennifer SotoDepartment of Bioengineering, University of California, Los Angeles, CA, 90095, USA.ORCID 0000-0002-0014-5213
Yang SongDepartment of Bioengineering, University of California, Los Angeles, CA, 90095, USA.
Yifan WuDepartment of Bioengineering, University of California, Los Angeles, CA, 90095, USA.
Binru ChenDepartment of Bioengineering, University of California, Los Angeles, CA, 90095, USA.
Hyungju ParkDepartment of Molecular and Cell Biology, University of California, Berkeley, CA, 94720, USA.
Navied AkhtarDepartment of Biomedical Engineering, University of California, Irvine, CA, 92617, USA.
Peng-Yuan WangDepartment of Bioengineering, University of California, Los Angeles, CA, 90095, USA.
Tyler HoffmanDepartment of Bioengineering, University of California, Los Angeles, CA, 90095, USA.
Chau LyDepartment of Bioengineering, University of California, Los Angeles, CA, 90095, USA.
Junren SiaDepartment of Bioengineering, University of California, Berkeley, CA, 94720, USA.
SzeYue WongDepartment of Bioengineering, University of California, Berkeley, CA, 94720, USA.
Douglas O KelkhoffDepartment of Bioengineering, University of California, Berkeley, CA, 94720, USA.
Julia ChuDepartment of Bioengineering, University of California, Berkeley, CA, 94720, USA.
Mu-Ming PooDepartment of Molecular and Cell Biology, University of California, Berkeley, CA, 94720, USA.
Timothy L DowningDepartment of Biomedical Engineering, University of California, Irvine, CA, 92617, USA.
Amy C RowatDepartment of Bioengineering, University of California, Los Angeles, CA, 90095, USA.
Song LiDepartment of Bioengineering, University of California, Los Angeles, CA, 90095, USA.ORCID 0000-0002-4760-8828

Funding

Women's CancersP30CA016042 · NCI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI Robert Damoiseaux · 1985 to 2026
$134.5M
Regenerative Musculoskeletal Medicine Training ProgramT32AR059033 · NIAMS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI John S Adams, Nicholas M. Bernthal · 2011 to 2026
$4.8M
Mechanopriming for cell engineeringR01NS130677 · NINDS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI Song Li · 2023 to 2026
$2.1M
Training Program in Cardiovascular Applied Research and EntrepreneurshipT32HL116270 · NHLBI · UNIVERSITY OF CALIFORNIA-IRVINE · PI HUGHES, CHRISTOPHER C. W. · 2013 to 2022
$1.6M
Regulation of cell reprogramming by matrix stiffnessR01GM143485 · NIGMS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI LI, SONG · 2021 to 2024
$1.3M
Pacific Biosciences PacBio RS Single Molecule Real Time SequencerS10OD018174 · OD · UNIVERSITY OF CALIFORNIA BERKELEY · PI ROKHSAR, DANIEL SOLEYMAN · 2014 to 2014
$600k
Multiphoton Microscope for Deep Tissue ImagingS10OD025017 · OD · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI BENTOLILA, LAURENT A. · 2018 to 2018
$599k
A Zeiss LSM 710 confocal microscope for the Biological Imaging Facility at the US10RR026866 · NCRR · UNIVERSITY OF CALIFORNIA BERKELEY · PI RUZIN, STEVEN EARL · 2010 to 2010
$419k
NIGMS NIH HHS R01 GM143485NIH HHS 1S10RR026866-01NIH HHS GM143485NIH HHS NIHS10OD018174NIH HHS NS130677NIH HHS P30CA016042NIH HHS S10 OD025017NIH HHS S10OD025017NIH HHS T32AR059033NIH HHS T32HL116270NINDS NIH HHS R01 NS130677
6 · The paper itself

Abstract

The role of transcription factors and biomolecules in cell type conversion has been widely studied. Yet, it remains unclear whether and how intracellular mechanotransduction through focal adhesions (FAs) and the cytoskeleton regulates the epigenetic state and cell reprogramming. Here, it is shown that cytoskeletal structures and the mechanical properties of cells are modulated during the early phase of induced neuronal (iN) reprogramming, with an increase in actin cytoskeleton assembly induced by Ascl1 transgene. The reduction of actin cytoskeletal tension or cell adhesion at the early phase of reprogramming suppresses the expression of mesenchymal genes, promotes a more open chromatin structure, and significantly enhances the efficiency of iN conversion. Specifically, reduction of intracellular tension or cell adhesion not only modulates global epigenetic marks, but also decreases DNA methylation and heterochromatin marks and increases euchromatin marks at the promoter of neuronal genes, thus enhancing the accessibility for gene activation. Finally, micro- and nano-topographic surfaces that reduce cell adhesions enhance iN reprogramming. These novel findings suggest that the actin cytoskeleton and FAs play an important role in epigenetic regulation for cell fate determination, which may lead to novel engineering approaches for cell reprogramming.

Indexed as

Cellular ReprogrammingEpigenesis, GeneticCell AdhesionChromatinMechanotransduction, CellularChromatincell adhesioncytoskeletondirect reprogrammingepigenetic state

Identifiers

PMID37357983
PMCPMC10460843

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.