ArticleNucleic acids research2023
Structural basis for high-affinity recognition of aflatoxin B1 by a DNA aptamer.
Article in Nucleic acids research, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 61 citations in OpenAlex.
- Nucleic acid aptamers: new methods for selection, target validation, molecular diagnostics and therapeutics.Signal transduction and targeted therapy · 2026Review
- Intracellular artificial DNA receptor-loaded gelated cells for aflatoxin theranostics.Nature communications · 2026Article
- Aflatoxin exposure and health impacts: global burden and advances in detection technologies.Genes and environment : the official journal of the Japanese Environmental Mutagen Society · 2026Review
- Reconsidering Molecular Docking Practices in Aptamer Research.Chembiochem : a European journal of chemical biology · 2026Article
- Flow-Through Aptamer-SERS Microfluidic Platform for Aflatoxin B1 Detection in Food Crops.ACS omega · 2026Article
- An electrochemiluminescence biosensor governed by a CRISPR-actuated electrostatic gate for ultrasensitive aflatoxin BMikrochimica acta · 2026Article
- A simple carbon-based electrochemical/photoelectrochemical dual-mode aptasensor for the detection of aflatoxin B1.Analytical and bioanalytical chemistry · 2026Article
- Single-stranded nucleic acid binding enhances the in vitro catalytic activity of Chikungunya virus nsP2 protease.BBA advances · 2026Article
- Aptamers-based wearable electrochemical sensors for continuous monitoring of biomarkers in vivo.Microsystems & nanoengineering · 2025Review
- Design and Characterization of Aptamers to Antibiotic Kanamycin with Improved Affinity.International journal of molecular sciences · 2025Article
- DNA Sequential Logic Circuits for Reversible Counters and Dynamic Biomolecular Sensing.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Silver coated porous silicon microarray SERS platform for detecting aflatoxin B1 fumonisin B1 and ochratoxin A.NPJ science of food · 2025Article
- Interactions of Ligand, Aptamer, and Complementary Oligonucleotide: Studying Impacts of NaMolecules (Basel, Switzerland) · 2025Article
- High Affinity Aptamers and Their Specificity for Azaspiracid-2 Using Capture-SELEX.Marine drugs · 2025Article
- Fluorescence assay for aflatoxin B1 based on aptamer-binding triggered DNAzyme activity.Analytical and bioanalytical chemistry · 2024Article
- Polycations as Aptamer-Binding Modulators for Sensitive Fluorescence Anisotropy Assay of Aflatoxin B1.Sensors (Basel, Switzerland) · 2024Article
- Recent Advances in Aptamer-Based Biosensors for Bacterial Detection.Biosensors · 2024Review
- Determination of Aflatoxin BFoods (Basel, Switzerland) · 2024Article
- Aptamers targeting SARS-CoV-2 nucleocapsid protein exhibit potential anti pan-coronavirus activity.Signal transduction and targeted therapy · 2024Article
- Review
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The 26-mer DNA aptamer (AF26) that specifically binds aflatoxin B1 (AFB1) with nM-level high affinity is rare among hundreds of aptamers for small molecules. Despite its predicted stem-loop structure, the molecular basis of its high-affinity recognition of AFB1 remains unknown. Here, we present the first high-resolution nuclear magnetic resonance structure of AFB1-AF26 aptamer complex in solution. AFB1 binds to the 16-residue loop region of the aptamer, inducing it to fold into a compact structure through the assembly of two bulges and one hairpin structure. AFB1 is tightly enclosed within a cavity formed by the bulges and hairpin, held in a place between the G·C base pair, G·G·C triple and multiple T bases, mainly through strong π-π stacking, hydrophobic and donor atom-π interactions, respectively. We further revealed the mechanism of the aptamer in recognizing AFB1 and its analogue AFG1 with only one-atom difference and introduced a single base mutation at the binding site of the aptamer to increase the discrimination between AFB1 and AFG1 based on the structural insights. This research provides an important structural basis for understanding high-affinity recognition of the aptamer, and for further aptamer engineering, modification and applications.
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