ArticleJournal of biomedical optics2023
Light-sheet autofluorescence lifetime imaging with a single-photon avalanche diode array.
Article in Journal of biomedical optics, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
21 citing papers in PubMed, 30 citations in OpenAlex.
- Segmentation-guided photon pooling enables robust single-cell analysis and fast fluorescence lifetime imaging microscopy.Journal of biomedical optics · 2026Article
- Label-free metabolic imaging monitors the fitness of chimeric antigen receptor T cells.Nature biomedical engineering · 2026Article
- Fluorescence Lifetime Microscopy Methods for Studying Dynamics of Fluorescent Proteins In Vivo and In Vitro.Advances in experimental medicine and biology · 2026Review
- Fluorescence Lifetime Imaging Techniques-A Review on Principles, Applications and Clinical Relevance.Journal of biophotonics · 2025Review
- Perspectives on label-free microscopy of heterogeneous and dynamic biological systems.Journal of biomedical optics · 2025Article
- Fast volumetric fluorescence lifetime imaging of multicellular systems using single-objective light-sheet microscopy.Communications biology · 2025Article
- Segmentation-guided photon pooling enables robust single cell analysis and fast fluorescence lifetime imaging microscopy.bioRxiv : the preprint server for biology · 2025Article
- High-speed fluorescence lifetime imaging microscopy: techniques, applications, and prospects.Biophotonics discovery · 2025Review
- Label-free metabolic fingerprinting of motile mammalian spermatozoa with subcellular resolution.BMC biology · 2025Article
- Cellpose as a reliable method for single-cell segmentation of autofluorescence microscopy images.Scientific reports · 2025Article
- Widefield frequency-domain autofluorescence lifetime imaging for detecting breast cancer in murine xenograft tumor tissues.Journal of biomedical optics · 2025Article
- Real-time wide-field fluorescence lifetime imaging via single-snapshot acquisition for biomedical applications.PhotoniX · 2025Article
- Overcoming photon and spatiotemporal sparsity in fluorescence lifetime imaging with SparseFLIM.Communications biology · 2024Article
- Imaging immunometabolismImmunometabolism (Cobham, Surrey) · 2024Article
- Cellpose as a reliable method for single-cell segmentation of autofluorescence microscopy images.bioRxiv : the preprint server for biology · 2024Article
- Advancements in fluorescence lifetime imaging microscopy Instrumentation: Towards high speed and 3D.Current opinion in solid state & materials science · 2024Article
- A Review of Image Sensors Used in Near-Infrared and Shortwave Infrared Fluorescence Imaging.Sensors (Basel, Switzerland) · 2024Review
- Single-sample image-fusion upsampling of fluorescence lifetime images.Science advances · 2024Article
- A time-correlated single photon counting SPAD array camera with a bespoke data-processing algorithm for lightsheet fluorescence lifetime imaging (FLIM) and FLIM videos.Scientific reports · 2024Article
- Probing organoid metabolism using fluorescence lifetime imaging microscopy (FLIM): The next frontier of drug discovery and disease understanding.Advanced drug delivery reviews · 2023Review
Corrections and comments
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Authors and funding
13 authors at 2 institutions in 1 country.
Funding
Abstract
Significance: Fluorescence lifetime imaging microscopy (FLIM) of the metabolic co-enzyme nicotinamide adenine dinucleotide (phosphate) [NAD(P)H] is a popular method to monitor single-cell metabolism within unperturbed, living 3D systems. However, FLIM of NAD(P)H has not been performed in a light-sheet geometry, which is advantageous for rapid imaging of cells within live 3D samples. Aim: We aim to design, validate, and demonstrate a proof-of-concept light-sheet system for NAD(P)H FLIM. Approach: A single-photon avalanche diode camera was integrated into a light-sheet microscope to achieve optical sectioning and limit out-of-focus contributions for NAD(P)H FLIM of single cells. Results: An NAD(P)H light-sheet FLIM system was built and validated with fluorescence lifetime standards and with time-course imaging of metabolic perturbations in pancreas cancer cells with 10 s integration times. NAD(P)H light-sheet FLIM Conclusions: FLIM of NAD(P)H is feasible in a light-sheet geometry and is attractive for 3D live cell imaging applications, such as monitoring immune cell metabolism and migration within an organism.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.