ArticleThoracic cancer2023
Silencing of circUSPL1 represses breast cancer progression by targeting miR-1296-5p/MTA1 axis.
Article in Thoracic cancer, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
2 citing papers in PubMed, 2 citations in OpenAlex.
- Circ_RPPH1 facilitates progression of breast cancer via miR-1296-5p/TRIM14 axis.Cancer biology & therapy · 2024Article
- Silencing of circUSPL1 represses breast cancer progression by targeting miR-1296-5p/MTA1 axis.Thoracic cancer · 2023Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe effect of circular RNAs (circRNAs) is widely studied in various human cancers, including breast cancer (BC). Herein, circUSPL1 has been recognized as a new regulator for BC progression. However, the detailed biological function and molecular mechanism of circUSPL1 in BC remain vague.
methodsThe expression level of circUSPL1, miR-1296-5p and metastasis associated 1 (MTA1) was examined by quantitative reverse transcription PCR. BC cell proliferation, migration, invasion, apoptosis and aerobic glycolysis were analyzed by colony formation assay, 5-ethynyl-2'-deoxyuridine assay, wound healing assay, transwell assay, flow cytometry and glycolysis corresponding kits, respectively. The protein level of Bcl-2, Bax, HK2, GLUT1 and MTA1 was evaluated by western blot analysis. The relationship of miR-1296-5p and circUSPL1 or MTA1 was affirmed using dual-luciferase reporter or RIP assays. A murine xenograft model was conducted to analyze the tumor growth in vivo.
resultsCircUSPL1 and MTA1 expression level was increased, but miR-1296-5p was particularly reduced in BC tissues and cells. CircUSPL1 deficiency significantly inhibited BC cell proliferation, migration, invasion, glycolysis, and promoted cell apoptosis. In addition, circUSPL1 directly targeted miR-1296-5p, and downregulation of miR-1296-5p eliminated the inhibitory action of circUSPL1 knockdown. Additionally, overexpression of miR-1296-5p repressed cell malignant properties, while the suppressive effects were overturned by MTA1 elevation. Lastly, silencing of circUSPL1 inhibited tumor growth by sponging miR-1296-5p and regulating MTA1.
conclusionCircUSPL1 deficiency repressed BC cell malignant phenotypes through reducing MTA1 via targeting miR-1296-5p, which might provide a theoretical basis for BC treatment.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.