Evidence map›Paper›PMID 37347284›Full record

ArticleCurrent genetics2023

TOF1 and RRM3 reveal a link between gene silencing and the pausing of replication forks.

Kholoud Shaban, Andrew Dolson, Ashley Fisher, Emma Lessard, Safia Mahabub Sauty, Krassimir Yankulov

Erratum issuedAbstract read
PubMed Publisher
In one paragraph

Article in Current genetics, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.2field-weighted citation impact, top 42% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Kholoud ShabanDepartment of Molecular and Cellular Biology, University of Guelph, Guelph, ON, N1G2W1, Canada.
Andrew DolsonDepartment of Molecular and Cellular Biology, University of Guelph, Guelph, ON, N1G2W1, Canada.
Ashley FisherDepartment of Molecular and Cellular Biology, University of Guelph, Guelph, ON, N1G2W1, Canada.
Emma LessardDepartment of Molecular and Cellular Biology, University of Guelph, Guelph, ON, N1G2W1, Canada.
Safia Mahabub SautyDepartment of Molecular and Cellular Biology, University of Guelph, Guelph, ON, N1G2W1, Canada.
Krassimir YankulovDepartment of Molecular and Cellular Biology, University of Guelph, Guelph, ON, N1G2W1, Canada. yankulov@uoguelph.ca.ORCID http://orcid.org/0000-0001-9440-9234
University of Guelph · CA

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Eukaryotic DNA replication is accompanied by the disassembly and reassembly of nucleosomes and the transmission of epigenetic marks to the newly assembled chromatids. Several histone chaperones, including CAF-1 and Asf1p, are central to these processes. On the other hand, replication forks pause at numerous positions throughout the genome, but it is not known if and how this pausing affects the reassembly and maintenance of chromatin structures. Here, we applied drug-free gene silencing assays to analyze the genetic interactions between CAC1, ASF1, and two genes that regulate the stability of the paused replisome (TOF1) and the resumption of elongation (RRM3). Our results show that TOF1 and RRM3 differentially interact with CAF-1 and ASF1 and that the deletions of TOF1 and RRM3 lead to reduced silencing and increased frequency of epigenetic conversions at three loci in the genome of S. cerevisiae. Our study adds details to the known activities of CAF-1 and Asf1p and suggests that the pausing of the replication fork can lead to epigenetic instability.

Indexed as

Saccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsCell Cycle ProteinsDNA-Binding ProteinsDNA HelicasesDNA ReplicationGene SilencingCell Cycle ProteinsDNA-Binding ProteinsDNA HelicasesSaccharomyces cerevisiae ProteinsTOF1 protein, S cerevisiaeASF1CAF-1Epigenetic conversionsReplication fork pausingRRM3TOF1

Identifiers

PMID37347284
OpenAlexW4381598783

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.