ArticleACS synthetic biology2023
In Vitro Transcription-Translation in an Artificial Biomolecular Condensate.
Article in ACS synthetic biology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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18 citing papers in PubMed, 34 citations in OpenAlex.
- Organization and Triggered Release of Liposomes with DNA-Based Synthetic Condensates.ACS nano · 2026Article
- Nanofluidics for Pioneering Synthetic Biology of Bottom-Up Cell-Free Molecular Systems.ACS synthetic biology · 2026Review
- Bottom-Up Coacervate-Based Artificial Cells: Integrating Cellular Hallmarks into Complex Life-Like Systems.Angewandte Chemie (International ed. in English) · 2026Review
- Dynamics of synthetic transcriptional condensates emerge from RNA synthesis and degradation.bioRxiv : the preprint server for biology · 2026Article
- Design and applications of synthetic biomolecular condensates.Nature nanotechnology · 2026Review
- Reconstituting alternative life using the test-bed of cell-free systems.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2025Review
- Dynamic Transcription Machineries in Protocells.Journal of the American Chemical Society · 2025Review
- Regulating Protein Immobilization During Cell-Free Protein Synthesis in Hyaluronan Microgels.Advanced biology · 2025Article
- Controlled and orthogonal partitioning of large particles into biomolecular condensates.Nature communications · 2025Article
- Metabolite-Responsive Control of Transcription by Phase Separation-Based Synthetic Organelles.ACS synthetic biology · 2025Article
- Chemically Triggered Reactive Coacervates Show Life-Like Budding and Membrane Formation.Journal of the American Chemical Society · 2025Article
- Cell-Free Gene Expression: Methods and Applications.Chemical reviews · 2025Review
- Membraneless protocell confined by a heat flow.Nature physics · 2025Article
- Shape transformations in peptide-DNA coacervates driven by enzyme-catalyzed deacetylation.Soft matter · 2024Article
- Nucleated synthetic cells with genetically driven intercompartment communication.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Template-based copying in chemically fuelled dynamic combinatorial libraries.Nature chemistry · 2024Article
- Controlled and orthogonal partitioning of large particles into biomolecular condensates.bioRxiv : the preprint server for biology · 2024Article
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Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Biomolecular condensates are a promising platform for synthetic cell formation and constitute a potential missing link between the chemical and cellular stage of the origins of life. However, it has proven challenging to integrate complex reaction networks into biomolecular condensates, such as a cell-free in vitro transcription-translation (IVTT) system. Integrating IVTT into biomolecular condensates successfully is one precondition for condensation-based synthetic cell formation. Moreover, it would provide a proof of concept that biomolecular condensates are in principle compatible with the central dogma, one of the hallmarks of cellular life. Here, we have systemically investigated the compatibility of eight different (bio)molecular condensates with IVTT incorporation. Of these eight candidates, we have found that a green fluorescent protein-labeled, intrinsically disordered cationic protein (GFP-K
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