ArticleApplications in plant sciences
A metabarcoding protocol targeting two DNA regions to analyze root-associated fungal communities in ferns and lycophytes.
Article in Applications in plant sciences. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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8 citing papers in PubMed, 13 citations in OpenAlex.
- Molecular markers for the study of arbuscular mycorrhizal fungi.Archives of microbiology · 2026Review
- Seasonal variation and drought effects on colonization and community structure of arbuscular mycorrhizal fungi and dark septate endophytes in desert plants.Frontiers in plant science · 2026Article
- The Influence of Agroecological Intensification on Dominant and Rare Microbial Communities Across Diverse European Countries.Microbial ecology · 2025Article
- Diversity of unique, nonmycorrhizal endophytic fungi in cultivatedPlant-environment interactions (Hoboken, N.J.) · 2024Article
- Fern mycorrhizae do not respond to fertilization in a tropical montane forest.Plant-environment interactions (Hoboken, N.J.) · 2024Article
- Facultative mycorrhization in a fern (Struthiopteris spicant L. Weiss) is bound to light intensity.BMC plant biology · 2024Article
- Comparative analysis of mycorrhizal communities associated withFrontiers in plant science · 2024Article
- A metabarcoding protocol targeting two DNA regions to analyze root-associated fungal communities in ferns and lycophytes.Applications in plant sciencesArticle
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Premise: Detailed studies of the fungi associated with lycophytes and ferns provide crucial insights into the early evolution of land plants. However, most investigations to date have assessed fern-fungus interactions based only on visual root inspection. In the present research, we establish and evaluate a metabarcoding protocol to analyze the fungal communities associated with fern and lycophyte roots. Methods: We used two primer pairs focused on the ITS rRNA region to screen the general fungal communities, and the 18S rRNA to target Glomeromycota fungi (i.e., arbuscular mycorrhizal fungi). To test these approaches, we collected and processed roots from 12 phylogenetically distant fern and lycophyte species. Results: We found marked compositional differences between the ITS and 18S data sets. While the ITS data set demonstrated the dominance of orders Glomerales (phylum Glomeromycota), Pleosporales, and Helotiales (both in phylum Ascomycota), the 18S data set revealed the greatest diversity of Glomeromycota. Non-metric multidimensional scaling (NMDS) ordination suggested an important geographical effect in sample similarities. Discussion: The ITS-based approach is a reliable and effective method to analyze the fungal communities associated with fern and lycophyte roots. The 18S approach is more appropriate for studies focused on the detailed screening of arbuscular mycorrhizal fungi.
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