ArticleApplications in plant sciences
Ethanol preservation and pretreatments facilitate quality DNA extractions in recalcitrant plant species.
Article in Applications in plant sciences. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 19 citations in OpenAlex.
- Combination of Sample Preservation Approaches and DNA Extraction Methods for Long-Read Sequencing of Nudibranchs' Genomes.Ecology and evolution · 2025Article
- Guidelines for the effective and ethical sampling of herbaria.Nature ecology & evolution · 2025Review
- Phylogenomic analyses support a new subgenus,Frontiers in plant science · 2025Article
- Versatile DNA extraction from diverse plant taxa using ionic liquids and magnetic ionic liquids: a methodological breakthrough for enhanced sample utility.Plant methods · 2024Article
- Comparative chloroplast genomes ofFrontiers in plant science · 2024Article
- Comparative and phylogenetic analyses of plastid genomes of the medicinally important genusFrontiers in plant science · 2024Article
- The complete chloroplast genomes of three Alismataceae species, including the medicinally importantMitochondrial DNA. Part B, Resources · 2024Article
- What is the "modified" CTAB protocol? Characterizing modifications to the CTAB DNA extraction protocol.Applications in plant sciencesArticle
- An optimized CTAB method for genomic DNA extraction from green seaweeds (Ulvophyceae).Applications in plant sciencesArticle
- Ethanol preservation and pretreatments facilitate quality DNA extractions in recalcitrant plant species.Applications in plant sciencesArticle
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Premise: The preservation of plant tissues in ethanol is conventionally viewed as problematic. Here, we show that leaf preservation in ethanol combined with proteinase digestion can provide high-quality DNA extracts. Additionally, as a pretreatment, ethanol can facilitate DNA extraction for recalcitrant samples. Methods: DNA was isolated from leaves preserved with 96% ethanol or from silica-desiccated leaf samples and herbarium fragments that were pretreated with ethanol. DNA was extracted from herbarium tissues using a special ethanol pretreatment protocol, and these extracts were compared with those obtained using the standard cetyltrimethylammonium bromide (CTAB) method. Results: DNA extracted from tissue preserved in, or pretreated with, ethanol was less fragmented than DNA from tissues without pretreatment. Adding proteinase digestion to the lysis step increased the amount of DNA obtained from the ethanol-pretreated tissues. The combination of the ethanol pretreatment with liquid nitrogen freezing and a sorbitol wash prior to cell lysis greatly improved the quality and yield of DNA from the herbarium tissue samples. Discussion: This study critically reevaluates the consequences of ethanol for plant tissue preservation and expands the utility of pretreatment methods for molecular and phylogenomic studies.
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