ArticleApplications in plant sciences
Modified CTAB protocols for high-molecular-weight DNA extractions from ferns.
Article in Applications in plant sciences. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed.
- Population and landscape genomics provide insights into the adaptive genetic variation and future climate-induced vulnerability of the endangered tree speciesHorticulture research · 2026Article
- Integrated GWAS and Fst analysis identify flowering-time regulatory genes in maize.The plant genome · 2026Article
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- Organellar phylogenomics of Ophioglossaceae fern genera.Frontiers in plant science · 2023Article
- What is the "modified" CTAB protocol? Characterizing modifications to the CTAB DNA extraction protocol.Applications in plant sciencesArticle
- Balancing read length and sequencing depth: Optimizing Nanopore long-read sequencing for monocots with an emphasis on the Liliales.Applications in plant sciencesArticle
- Optimizing DNA extraction protocols for bryophytes: Insights from Orthotrichaceae.Applications in plant sciencesArticle
- Modified CTAB protocols for high-molecular-weight DNA extractions from ferns.Applications in plant sciencesArticle
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Authors and funding
3 authors.
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Abstract
Premise: Efficient protocols for extracting high-molecular-weight (HMW) DNA from ferns facilitate the long-read sequencing of their large and complex genomes. Here, we perform two cetyltrimethylammonium bromide (CTAB)-based protocols to extract HMW DNA and evaluate their applicability in diverse fern taxa for the first time. Methods and Results: We describe two modified CTAB protocols, with key adjustments to minimize mechanical disruption during lysis to prevent DNA shearing. One of these protocols uses a small amount of fresh tissue but yields a considerable quantity of HMW DNA with high efficiency. The other accommodates a large amount of input tissue, adopts an initial step of nuclei isolation, and thus ensures a high yield in a short period of time. Both methods were proven to be robust and effective in obtaining HMW DNA from diverse fern lineages, including 33 species in 19 families. The DNA extractions mostly had high DNA integrity, with mean sizes larger than 50 kbp, as well as high purity (A Conclusions: This study provides HMW DNA extraction protocols for ferns in the hope of facilitating further attempts to sequence their genomes, which will bridge our genomic understanding of land plant diversity.
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