ArticleApplications in plant sciences
Optimizing the lysis step in CTAB DNA extractions of silica-dried and herbarium leaf tissues.
Article in Applications in plant sciences. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- A dataset of 352 nuclear genes for accurate species identification and geographical origin traceability ofData in brief · 2026Article
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- Complete chloroplast genomes of four Triumfetta species obtained from herbarium specimens: comparative analysis and identification of a synapomorphic inversion.Scientific reports · 2026Article
- Joint transcriptomic and metabolomic analyses of the mechanisms ofAoB PLANTS · 2026Article
- A chloroplast genomic dataset for accurate identification of the endangeredData in brief · 2026Article
- Comparative performance of traditional and commercial DNA extraction methods for fish gut microbiota analysis.Molecular biology reports · 2026Article
- Comparative chloroplast genomics reveals codon usage bias, simple sequence repeat dynamics, and phylogenomic relationships in Urticaceae.Frontiers in plant science · 2026Article
- Article
- Sample Preparation for Multi-Omics Analysis: Considerations and Guidance for Identifying the Ideal Workflow.Proteomics · 2025Review
- Improved genomic DNA extraction from citrus species using a modified CTAB method.Molecular biology reports · 2025Article
- Reducing costs and shortening the cetyltrimethylammonium bromide (CTAB) method to improve DNA extraction efficiency from wintersweet and some other plants.Scientific reports · 2025Article
- Exploring the potential role of EPSPS mutations for enhanced glyphosate resistance inFrontiers in plant science · 2025Article
- Identification of candidate genes and development of KASP markers for soybean pod-related traits using GWAS.Frontiers in plant science · 2025Article
- Comparison of Three Methods to Extract Plasmodium falciparum DNA from Whole Blood and Dried Blood Spots.The American journal of tropical medicine and hygiene · 2024Article
- Complete Chloroplast Genomes and the Phylogenetic Analysis of Three Native Species of Paeoniaceae from the Sino-Himalayan Flora Subkingdom.International journal of molecular sciences · 2023Article
- Genetic diversity and population structure of Polygonatum cyrtonema Hua in China using SSR markers.PloS one · 2023Article
- Optimizing DNA extraction protocols for bryophytes: Insights from Orthotrichaceae.Applications in plant sciencesArticle
- What is the "modified" CTAB protocol? Characterizing modifications to the CTAB DNA extraction protocol.Applications in plant sciencesArticle
- An optimized CTAB method for genomic DNA extraction from green seaweeds (Ulvophyceae).Applications in plant sciencesArticle
- Optimizing the lysis step in CTAB DNA extractions of silica-dried and herbarium leaf tissues.Applications in plant sciencesArticle
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4 authors.
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Abstract
Premise: The use of cetyltrimethylammonium bromide (CTAB) is an effective and inexpensive method of extracting DNA from plants. The CTAB protocol is frequently modified to optimize DNA extractions, but experimental approaches rarely perturb a single variable at a time to systematically infer their effect on DNA quantity and quality. Methods and Results: We investigated how chemical additives, incubation temperature, and lysis duration affected DNA quantity and quality. Altering those parameters influenced DNA concentrations and fragment lengths, but only extractant purity was significantly affected. CTAB and CTAB plus polyvinylpyrrolidone buffers produced the highest DNA quality and quantity. Extractions from silica gel-preserved tissues had significantly higher DNA yield, longer DNA fragments, and purer extractants compared to herbarium-preserved tissues. Conclusions: We recommend DNA extractions of silica gel-preserved tissues that include a shorter and cooler lysis step, which results in purer extractions compared to a longer and hotter lysis step, while preventing fragmentation and reducing time.
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