ArticleApplications in plant sciences
What is the "modified" CTAB protocol? Characterizing modifications to the CTAB DNA extraction protocol.
Article in Applications in plant sciences. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 82 papers.
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82 citing papers in PubMed.
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- A high-quality chromosome-level genome assembly of Ficus carica cultivar 'Green Peel'.Scientific data · 2026Article
- Genetic Diversity and SNP-Based Fingerprinting of 94 Pumpkin Cultivars: Database Establishment and Population Analysis.Plants (Basel, Switzerland) · 2026Article
- A rapid Agrobacterium rhizogenes-mediated transient expression for assessing sgRNA efficiency in CRISPR-Act3.0 in tomato.Plant cell reports · 2026Article
- Revealing novelty from the southwestern Atlantic, Yemanjia gen. nov. and Olokunococcus gen. nov. from the coral cyanobiome of the Abrolhos Bank.Journal of phycology · 2026Article
- Construction and Biological Characterization of Papaya Leaf Curl Guangdong Virus Infectious cDNA Clone.Microbial biotechnology · 2026Article
- Plastid genome structure and phylogenomics of the freshwater red algal order Batrachospermales (Rhodophyta).Journal of phycology · 2026Article
- Electrochemical Biosensing Platforms for Rapid and Early Diagnosis of Crop Fungal and Viral Diseases.Sensors (Basel, Switzerland) · 2026Review
- Haplotype-resolved T2T gap-free genomes of the winegrape cultivar Cabernet Sauvignon.Scientific data · 2026Article
- Phylotranscriptomics and genome-size evidence clarify the Taiwanese Cirsium japonicum complex and delimit C. brevicaule and allied East Asian thistles.BMC plant biology · 2026Article
- Natural variation in VrERF088 modulates drought tolerance during mungbean germination.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2026Article
- A TaqMan Real-Time PCR Assay for Early and Accurate Detection of Hypomontagnella monticulosa, an Emerging Pathogen Causing White Leaf Spot on Pachira glabra Pasq.The plant pathology journal · 2026Article
- Rapid Identification of Candidate SNPs and QTLs forCurrent issues in molecular biology · 2026Article
- Cutting through the clones: genomic strategies for core collection development in moso bamboo.BMC genomics · 2026Article
22 more citing papers are in PubMed but not listed here.
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4 authors.
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Abstract
Cetyltrimethylammonium bromide (CTAB)-based methods are widely used to isolate DNA from plant tissues, but the unique chemical composition of secondary metabolites among plant species has necessitated optimization. Research articles often cite a "modified" CTAB protocol without explicitly stating how the protocol had been altered, creating non-reproducible studies. Furthermore, the various modifications that have been applied to the CTAB protocol have not been rigorously reviewed and doing so could reveal optimization strategies across study systems. We surveyed the literature for modified CTAB protocols used for the isolation of plant DNA. We found that every stage of the CTAB protocol has been modified, and we summarized those modifications to provide recommendations for extraction optimization. Future genomic studies will rely on optimized CTAB protocols. Our review of the modifications that have been used, as well as the protocols we provide here, could better standardize DNA extractions, allowing for repeatable and transparent studies.
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