ArticleApplications in plant sciences
High-molecular-weight DNA extraction for long-read sequencing of plant genomes: An optimization of standard methods.
Article in Applications in plant sciences. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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15 citing papers in PubMed, 25 citations in OpenAlex.
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- Optimizing DNA extraction protocols for bryophytes: Insights from Orthotrichaceae.Applications in plant sciencesArticle
- What is the "modified" CTAB protocol? Characterizing modifications to the CTAB DNA extraction protocol.Applications in plant sciencesArticle
- Balancing read length and sequencing depth: Optimizing Nanopore long-read sequencing for monocots with an emphasis on the Liliales.Applications in plant sciencesArticle
- High-molecular-weight DNA extraction for long-read sequencing of plant genomes: An optimization of standard methods.Applications in plant sciencesArticle
- A cost-effective DNA extraction protocol for long-read sequencing in non-model plants.Applications in plant sciencesArticle
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Premise: Developing an effective and easy-to-use high-molecular-weight (HMW) DNA extraction method is essential for genomic research, especially in the era of third-generation sequencing. To efficiently use technologies capable of generating long-read sequences, it is important to maximize both the length and purity of the extracted DNA; however, this is frequently difficult to achieve with plant samples. Methods and Results: We present a HMW DNA extraction method that combines (1) a nuclei extraction method followed by (2) a traditional cetyltrimethylammonium bromide (CTAB) DNA extraction method for plants with optimized extraction conditions that influence HMW DNA recovery. Our protocol produced DNA fragments (percentage of fragments >20 kbp) that were, on average, ca. five times longer than those obtained using a commercial kit, and contaminants were removed more effectively. Conclusions: This effective HMW DNA extraction protocol can be used as a standard protocol for a diverse array of taxa, which will enhance plant genomic research.
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