Evidence map›Paper›PMID 37340198›Full record

ReviewMolecular biotechnology2024

Agrobacterium tumefaciens-Mediated Plant Transformation: A Review.

Shahnam Azizi-Dargahlou, Mahin Pouresmaeil

Abstract readReview
PubMed Publisher
In one paragraph

Review in Molecular biotechnology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 54 papers.

0numbers the graph read from it
0cells of the map it votes in
54citing papers in PubMed
14.8field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

54 citing papers in PubMed, 97 citations in OpenAlex.

  1. Review
  2. Article
  3. Review
  4. Review
  5. Review
  6. Efficient Embryogenic Callus Induction andPlants (Basel, Switzerland) · 2026
    Article
  7. Genome Editing Approaches in Flax (International journal of molecular sciences · 2026
    Review
  8. Review
  9. Review
  10. Article
  11. Generation and Characterization of a Genetically ModifiedInternational journal of molecular sciences · 2026
    Article
  12. Article
  13. Establishment of an efficientFrontiers in plant science · 2026
    Article
  14. Article
  15. Review
  16. Functional Analysis of Naturally IntegratedPlants (Basel, Switzerland) · 2025
    Article
  17. Review
  18. Microorganisms · 2025
    Review
  19. Article
  20. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors at 1 institution in 1 country.

Shahnam Azizi-DargahlouDepartment of Biotechnology, Azarbaijan Shahid Madani University, Tabriz, Iran. shahnambio@gmail.com.ORCID http://orcid.org/0000-0002-7658-5135
Mahin PouresmaeilDepartment of Biotechnology, Azarbaijan Shahid Madani University, Tabriz, Iran.
Azarbaijan Shahid Madani University · IR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Agrobacterium tumefaciens-mediated plant transformation is the most dominant technique for the transformation of plants. It is used to transform monocotyledonous and dicotyledonous plants. A. tumefaciens apply for stable and transient transformation, random and targeted integration of foreign genes, as well as genome editing of plants. The Advantages of this method include cheapness, uncomplicated operation, high reproducibility, a low copy number of integrated transgenes, and the possibility of transferring larger DNA fragments. Engineered endonucleases such as CRISPR/Cas9 systems, TALENs, and ZFNs can be delivered with this method. Nowadays, Agrobacterium-mediated transformation is used for the Knock in, Knock down, and Knock out of genes. The transformation effectiveness of this method is not always desirable. Researchers applied various strategies to improve the effectiveness of this method. Here, a general overview of the characteristics and mechanism of gene transfer with Agrobacterium is presented. Advantages, updated data on the factors involved in optimizing this method, and other useful materials that lead to maximum exploitation as well as overcoming obstacles of this method are discussed. Moreover, the application of this method in the generation of genetically edited plants is stated. This review can help researchers to establish a rapid and highly effective Agrobacterium-mediated transformation protocol for any plant species.

Indexed as

Agrobacterium tumefaciensPlants, Genetically ModifiedTransformation, GeneticCRISPR-Cas SystemsGene EditingGene Transfer TechniquesTransgenesEngineered endonucleasesGenome editingPlant transformationTransgenic plantsTransient and stable expression

Identifiers

PMID37340198
OpenAlexW4381329355

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.