ArticleScientific reports2023
A PETase enzyme synthesised in the chloroplast of the microalga Chlamydomonas reinhardtii is active against post-consumer plastics.
Article in Scientific reports, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 33 citations in OpenAlex.
- Plastic-algae interactions in freshwater and marine systems: mechanisms, evidence and prospects for bioremediation.World journal of microbiology & biotechnology · 2026Review
- Engineering bidirectional chloroplast promoters for tunable co-expression of multiple genes in microalgae (Chlamydomonas reinhardtii).Communications biology · 2026Article
- The effect of ionic strength on PETase enzymes: An experimental and computational study.Protein science : a publication of the Protein Society · 2026Article
- Lignin-Sourced Aromatics for Biodegradable Flexible Copolyesters Mimicking Poly(Butylene Adipate-co- Terephthalate).ChemSusChem · 2025Article
- Engineering of Synthetic Microbial Consortia for Sustainable Management of Wastewater and Polyethylene Terephthalate: A Comprehensive Review.International journal of molecular sciences · 2025Review
- EngineeringInternational journal of molecular sciences · 2025Review
- Efficient secretion of a plastic degrading enzyme from the green algae Chlamydomonas reinhardtii.Scientific reports · 2025Article
- Functional and Structural Characterization of PETase SM14 from Marine-Sponge Streptomyces sp. Active on Polyethylene Terephthalate.ACS sustainable chemistry & engineering · 2025Article
- Further characterization and engineering of an 11-amino acid motif for enhancing recombinant soluble protein expression.Microbial cell factories · 2025Article
- Review
- Efficient biodegradation and upcycling of polyethylene terephthalate mediated by cell-factories.Frontiers in microbiology · 2025Review
- A critical review of microplastics in aquatic ecosystems: Degradation mechanisms and removing strategies.Environmental science and ecotechnology · 2024Review
- The MicroalgaeCells · 2024Review
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Polyethylene terephthalate hydrolases (PETases) are a newly discovered and industrially important class of enzymes that catalyze the enzymatic degradation of polyethylene terephatalate (PET), one of the most abundant plastics in the world. The greater enzymatic efficiencies of PETases compared to close relatives from the cutinase and lipase families have resulted in increasing research interest. Despite this, further characterization of PETases is essential, particularly regarding their possible activity against other kinds of plastic. In this study, we exploited for the first time the use of the microalgal chloroplast for more sustainable synthesis of a PETase enzyme. A photosynthetic-restoration strategy was used to generate a marker-free transformant line of the green microalga Chlamydomonas reinhardtii in which the PETase from Ideonella sakaiensis was constitutively expressed in the chloroplast. Subsequently, the activity of the PETase against both PET and post-consumer plastics was investigated via atomic force microscopy, revealing evidence of degradation of the plastics.
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