ArticleNature communications2023
Discovery of extended product structural space of the fungal dioxygenase AsqJ.
Article in Nature communications, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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Who cites it
4 citing papers in PubMed.
- Substrate Epoxidation Catalyzed by the Nonheme Iron Dioxygenase Dapdiamide Biosynthesis Enzyme C. Why Is the Substrate Tethered?Chemistry (Weinheim an der Bergstrasse, Germany) · 2025Article
- Enzymatic Peroxidation in the Chemoenzymatic Synthesis of 13-Oxoverruculogen.Synlett : accounts and rapid communications in synthetic organic chemistry · 2025Article
- Product Selectivity in Baeyer-Villiger Monooxygenase-Catalyzed Bacterial Alkaloid Core Structure Maturation.Journal of the American Chemical Society · 2024Article
- Chemoenzymatic Synthesis of 13-Oxoverruculogen.Journal of the American Chemical Society · 2023Article
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The fungal dioxygenase AsqJ catalyses the conversion of benzo[1,4]diazepine-2,5-diones into quinolone antibiotics. A second, alternative reaction pathway leads to a different biomedically important product class, the quinazolinones. Within this work, we explore the catalytic promiscuity of AsqJ by screening its activity across a broad range of functionalized substrates made accessible by solid-/liquid-phase peptide synthetic routes. These systematic investigations map the substrate tolerance of AsqJ within its two established pathways, revealing significant promiscuity, especially in the quinolone pathway. Most importantly, two further reactivities leading to new AsqJ product classes are discovered, thus significantly expanding the structural space accessible by this biosynthetic enzyme. Switching AsqJ product selectivity is achieved by subtle structural changes on the substrate, revealing a remarkable substrate-controlled product selectivity in enzyme catalysis. Our work paves the way for the biocatalytic synthesis of diverse biomedically important heterocyclic structural frameworks.
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