Evidence map›Paper›PMID 37339797›Full record

ArticleJournal for immunotherapy of cancer2023

Method for estimation of apoptotic cell fraction of cytotherapy using in vivo fluorine-19 magnetic resonance: pilot study in a patient with head and neck carcinoma receiving tumor-infiltrating lymphocytes labeled with perfluorocarbon nanoemulsion.

Eric T Ahrens, Brooke M Helfer, Charles F O'Hanlon, Deanne R Lister, Julie L Bykowski, Karen Messer, Benjamin I Leach, Jiawen Chen, Hongyan Xu, Gregory A Daniels and 1 more

Erratum issuedOpen access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal for immunotherapy of cancer, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
0.7field-weighted citation impact, top 35% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 7 citations in OpenAlex.

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4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

11 authors at 2 institutions in 1 country.

Eric T AhrensDepartment of Radiology, University of California San Diego, La Jolla, California, USA eahrens@ucsd.edu.ORCID 0000-0001-6131-9304
Brooke M HelferCelsense, Pittsburgh, Pennsylvania, USA.
Charles F O'HanlonLot 49 Consulting, Pittsburgh, Pennsylvania, USA.
Deanne R ListerDepartment of Radiology, University of California San Diego, La Jolla, California, USA.
Julie L BykowskiDepartment of Radiology, University of California San Diego, La Jolla, California, USA.
Karen MesserDivision of Biostatistics, University of California San Diego, La Jolla, California, USA.
Benjamin I LeachDepartment of Radiology, University of California San Diego, La Jolla, California, USA.
Jiawen ChenDepartment of Electrical and Computer Engineering, University of California San Diego, La Jolla, California, USA.
Hongyan XuDepartment of Radiology, University of California San Diego, La Jolla, California, USA.
Gregory A DanielsDepartment of Medicine, University of California San Diego, La Jolla, California, USA.
Ezra E W CohenDepartment of Medicine, University of California San Diego, La Jolla, California, USA.
University of California San Diego · USCelsense (United States) · US

Funding

CLINICAL TRANSLATION OF 19F MRI TO VISUALIZE CANCER IMMUNOTHERAPEUTIC CELLSR01CA134633 · NCI · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI AHRENS, ERIC T., COHEN, EZRA · 2009 to 2021
$4.4M
NCI NIH HHS R01 CA134633
6 · The paper itself

Abstract

backgroundAdoptive transfer of T cells is a burgeoning cancer therapeutic approach. However, the fate of the cells, once transferred, is most often unknown. We describe the first clinical experience with a non-invasive biomarker to assay the apoptotic cell fraction (ACF) after cell therapy infusion, tested in the setting of head and neck squamous cell carcinoma (HNSCC). A patient with HNSCC received autologous tumor-infiltrating lymphocytes (TILs) labeled with a perfluorocarbon (PFC) nanoemulsion cell tracer. Nanoemulsion, released from apoptotic cells, clears through the reticuloendothelial system, particularly the Kupffer cells of the liver, and fluorine-19 (

methodsAutologous TILs were isolated from a patient in their late 50s with relapsed, refractory human papillomavirus-mediated squamous cell carcinoma of the right tonsil, metastatic to the lung. A lung metastasis was resected for T cell harvest and expansion using a rapid expansion protocol. The expanded TILs were intracellularly labeled with PFC nanoemulsion tracer by coincubation in the final 24 hours of culture, followed by a wash step. At 22 days after intravenous infusion of TILs, quantitative single-voxel liver

resultsWe show that it is feasible to PFC-label ~70×10

conclusionsSurvival of the primary cell therapy product is likely to vary per patient. A non-invasive assay of ACF over time could potentially provide insight into the mechanisms of response and non-response, informing future clinical studies. This information may be useful to developers of cytotherapies and clinicians as it opens an avenue to quantify cellular product survival and engraftment.

Indexed as

Carcinoma, Squamous CellFluorocarbonsHead and Neck NeoplasmsApoptosisFluorineHumansLymphocytes, Tumor-InfiltratingMagnetic Resonance ImagingMagnetic Resonance SpectroscopyPilot ProjectsSquamous Cell Carcinoma of Head and NeckFluorineFluorocarbonsImmunologic TechniquesImmunotherapyLymphocytes, Tumor-InfiltratingT-Lymphocytes

Identifiers

PMID37339797
PMCPMC10314637
OpenAlexW4381435356

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.