ArticleThe Plant cell2023
Arabidopsis PLANT U-BOX44 down-regulates osmotic stress signaling by mediating Ca2+-DEPENDENT PROTEIN KINASE4 degradation.
Article in The Plant cell, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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9 citing papers in PubMed, 20 citations in OpenAlex.
- The CPK3-mediated cytosolic-nuclear translocation of bHLH107 recruits HY5 to regulate the CuJournal of advanced research · 2026Article
- Phytochrome B integrates jasmonic acid and warm temperature signaling pathways to regulate cotyledon chloroplast development.Nature communications · 2026Article
- Identification and characterization of U-box E3 ubiquitin ligase genes (ChPUBs) in Cardamine hupingshanensis under abiotic stress.BMC genomics · 2026Article
- Exploring the molecular landscape of environmental responses in the Antarctic plantFrontiers in plant science · 2026Article
- Development and selection of stably expressed reference genes for expression normalization in Ribes odoratum under drought stress.Scientific reports · 2025Article
- Genome-Wide Identification, Characterization, and Expression Analysis of the U-Box Gene Family in Cucumber (Plants (Basel, Switzerland) · 2025Article
- E3 Ubiquitin Ligase OsRFI2 Regulates Salinity Tolerance by Targeting Ascorbate Peroxidase OsAPX8 for its Degradation in Rice.Rice (New York, N.Y.) · 2025Article
- RsRbohD1 Plays a Significant Role in ROS Production during Radish Pithiness Development.Plants (Basel, Switzerland) · 2024Article
- Mind the pretzels and the pint: The PUB keeps you thirsty.The Plant cell · 2023Article
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6 authors at 2 institutions in 2 countries.
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Abstract
Calcium (Ca2+)-dependent protein kinases (CPKs) are essential regulators of plant responses to diverse environmental stressors, including osmotic stress. CPKs are activated by an increase in intracellular Ca2+ levels triggered by osmotic stress. However, how the levels of active CPK protein are dynamically and precisely regulated has yet to be determined. Here, we demonstrate that NaCl/mannitol-induced osmotic stress promoted the accumulation of CPK4 protein by disrupting its 26S proteasome-mediated CPK4 degradation in Arabidopsis (Arabidopsis thaliana). We isolated PLANT U-BOX44 (PUB44), a U-box type E3 ubiquitin ligase that ubiquitinates CPK4 and triggers its degradation. A calcium-free or kinase-inactive CPK4 variant was preferentially degraded compared to the Ca2+-bound active form of CPK4. Furthermore, PUB44 exhibited a CPK4-dependent negative role in the response of plants to osmotic stress. Osmotic stress induced the accumulation of CPK4 protein by inhibiting PUB44-mediated CPK4 degradation. The present findings reveal a mechanism for regulating CPK protein levels and establish the relevance of PUB44-dependent CPK4 regulation in modulating plant osmotic stress responses, providing insights into osmotic stress signal transduction mechanisms.
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