Evidence map›Paper›PMID 37336818›Full record

ArticleMikrochimica acta2023

Rapid and sensitive detection of E. coli O157:H7 by lateral flow immunoassay and silver enhancement.

Shayesteh Bazsefidpar, Esther Serrano-Pertierra, Gemma Gutiérrez, Alberto Sánchez Calvo, María Matos, María Carmen Blanco-López

Open access · hybridAbstract read
In one paragraph

Article in Mikrochimica acta, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
3.2field-weighted citation impact, top 8% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 29 citations in OpenAlex.

  1. Review
  2. Article
  3. Recent Advancements in the SERS-Based Detection ofSensors (Basel, Switzerland) · 2026
    Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Shayesteh BazsefidparDepartment of Physical and Analytical Chemistry & Institute of Biotechnology of Asturias, University of Oviedo, c/Julián Clavería 8, 33006, Oviedo, Spain.
Esther Serrano-PertierraDepartment of Physical and Analytical Chemistry & Institute of Biotechnology of Asturias, University of Oviedo, c/Julián Clavería 8, 33006, Oviedo, Spain.
Gemma GutiérrezDepartment of Chemical and Environmental Engineering & Institute of Biotechnology of Asturias, University of Oviedo, Oviedo, Spain.
Alberto Sánchez CalvoDepartment of Physical and Analytical Chemistry & Institute of Biotechnology of Asturias, University of Oviedo, c/Julián Clavería 8, 33006, Oviedo, Spain.
María MatosDepartment of Chemical and Environmental Engineering & Institute of Biotechnology of Asturias, University of Oviedo, Oviedo, Spain.
María Carmen Blanco-LópezDepartment of Physical and Analytical Chemistry & Institute of Biotechnology of Asturias, University of Oviedo, c/Julián Clavería 8, 33006, Oviedo, Spain. cblanco@uniovi.es.ORCID http://orcid.org/0000-0002-9776-9013
Universidad de Oviedo · ES

Funding

Consejería de Educación y Ciencia del Principado de Asturias (Spain) SV-PA-21-AYUD/2021/52132European Commission 813439
6 · The paper itself

Abstract

The aim of this study was to develop a sensitive lateral flow immunoassay (LFIA) for the rapid detection of Escherichia coli (E. coli) O157:H7, a pathogen contributor to diseases and fatalities worldwide. Au nanoparticles with high stability, uniform size, and shape were synthesized and coated with heterobifunctional PEG polymer with carboxyl groups, and they were bioconjugated to be used as label in sandwich-LFIA. Then, a silver enhancement strategy was developed as an accessible, rapid, and cost-effective approach for signal amplification to reduce the limit of detection (LOD). The optimal results were achieved when a solution of silver nitrate and hydroquinone/citrate buffer was added to the strips for 4 min. This led to a decrease in the visual LOD from 2 × 10

Indexed as

Escherichia coli O157Metal NanoparticlesFood MicrobiologyGoldImmunoassayReproducibility of ResultsGoldE. coli O157:H7Food analysisFoodborne pathogensPEG polymerSandwich-LFIASilver enhancement

Identifiers

PMID37336818
PMCPMC10279566
OpenAlexW4381187988

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.