Evidence map›Paper›PMID 37334357›Full record

ArticleFrontiers in immunology2023

Increasing human monoclonal antibody cloning efficiency with a whole-cell modified immunoglobulin-capture assay (mICA).

Sara Siris, Camilla A Gladstone, Yanping Guo, Radhika Patel, Christopher L Pinder, Robin J Shattock, Paul F McKay, Paul R Langford, Fadil A Bidmos

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Frontiers in immunology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.2field-weighted citation impact, top 43% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 1 institution in 1 country.

Sara SirisSection of Paediatric Infectious Disease, Department of Infectious Disease, Imperial College London, London, United Kingdom.
Camilla A GladstoneSection of Paediatric Infectious Disease, Department of Infectious Disease, Imperial College London, London, United Kingdom.
Yanping GuoFlow Cytometry Core Facility, National Heart and Lung Institute, Imperial College London, London, United Kingdom.
Radhika PatelFlow Cytometry Core Facility, National Heart and Lung Institute, Imperial College London, London, United Kingdom.
Christopher L PinderSection of Virology, Department of Infectious Disease, Imperial College London, London, United Kingdom.
Robin J ShattockSection of Virology, Department of Infectious Disease, Imperial College London, London, United Kingdom.
Paul F McKaySection of Virology, Department of Infectious Disease, Imperial College London, London, United Kingdom.
Paul R LangfordSection of Paediatric Infectious Disease, Department of Infectious Disease, Imperial College London, London, United Kingdom.
Fadil A BidmosSection of Paediatric Infectious Disease, Department of Infectious Disease, Imperial College London, London, United Kingdom.
Imperial College London · GB

Funding

Medical Research Council MR/S007490/1
6 · The paper itself

Abstract

Expression cloning of fully human monoclonal antibodies (hmAbs) is seeing powerful utility in the field of vaccinology, especially for elucidating vaccine-induced B-cell responses and novel vaccine candidate antigen discovery. Precision of the hmAb cloning process relies on efficient isolation of hmAb-producing plasmablasts of interest. Previously, a novel immunoglobulin-capture assay (ICA) was developed, using single protein vaccine antigens, to enhance the pathogen-specific hmAb cloning output. Here, we report a novel modification of this single-antigen ICA using formalin-treated, fluorescently stained whole cell suspensions of the human bacterial invasive pathogens,

Indexed as

Antibodies, MonoclonalMeningococcal VaccinesAntigens, BacterialBacterial VaccinesCloning, MolecularHumansPneumococcal VaccinesStreptococcus pneumoniaeSuspensionsAntibodies, MonoclonalAntigens, BacterialBacterial VaccinesMeningococcal VaccinesPneumococcal VaccinesSuspensionsB-cellhuman monoclonal antibodiesNeisseria meningitidisplasmablast enrichmentreverse vaccinology 2.0Streptococcus pneumoniaevaccines

Identifiers

PMID37334357
PMCPMC10272928
OpenAlexW4379231516

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.