Development of RT-qPCR for quantification of human enterovirus D68

Hanne Lillerovde Ørstenvik, Ann-Kristin Tveten, Yanran Cao

Open access · goldAbstract read

In one paragraph

Article in MethodsX, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it

0cells of the map it votes in

1citing papers in PubMed

0.2field-weighted citation impact, top 44% of its field

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

Tools and approaches to study the human gut virome: from the bench to bioinformatics.mSystems · 2026
Review

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Hanne Lillerovde ØrstenvikFaculty of Natural Sciences, Department of Biological Sciences Ålesund, Norwegian University of Science and Technology, Norway.

Ann-Kristin TvetenFaculty of Natural Sciences, Department of Biological Sciences Ålesund, Norwegian University of Science and Technology, Norway.

Yanran CaoFaculty of Natural Sciences, Department of Biological Sciences Ålesund, Norwegian University of Science and Technology, Norway.

Norwegian University of Science and Technology · NO

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The common cold is the most frequent viral infectious disease of the upper respiratory tract with different intensities based on the serotype and the characteristics of the virus. Numerous human rhinoviruses have been identified and classified. Human rhinovirus 87 (HRV87), also known as enterovirus D68 (EV-D68), is one of the common viruses causing respiratory infections. In this study, a reverse transcription-quantitative polymerase chain reaction (RT-qPCR) assay was developed, optimized, and validated for the detection of EV-D68. Method development also covers specificity, sensitivity, efficiency, and inter-and-intra-assay variations. Overall, this one-step qPCR assay will permit quantitative assessments of human enterovirus D68 RNA.•Enterovirus D68 is a reemerging viral agent causing respiratory infection.•RT-qPCR assay developed for detection of human enterovirus D68.•In this article validation to secure reproducibility is done according to MIQE guidelines.

Indexed as

DetectionEnterovirus D68Human Rhinovirus 87QuantificationReal-time PCRRespiratory infectionReverse transcription-quantitative polymerase chain reaction (RT-qPCR) assay

Identifiers

PMID37333509

DOI10.1016/j.mex.2023.102234

PMCPMC10272510

OpenAlexW4378906388

What OpenQuestion holds

Textmetadata

LicenceCC BY

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.