Evidence map›Paper›PMID 37333364›Full record

ArticlemedRxiv : the preprint server for health sciences2023

Rapid and Multiplexed Nucleic Acid Detection using Programmable Aptamer-Based RNA Switches.

Zhaoqing Yan, Anli A Tang, Amit Eshed, Zackary M Ticktin, Soma Chaudhary, Duo Ma, Griffin McCutcheon, Yudan Li, Kaiyue Wu, Sanchari Saha and 6 more

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in medRxiv : the preprint server for health sciences, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

16 authors.

Zhaoqing YanDepartment of Biomedical Engineering, Boston University, Boston, MA, USA.
Anli A TangBiodesign Center for Molecular Design and Biomimetics at the Biodesign Institute, Arizona State University, Tempe, AZ, USA.
Amit EshedDepartment of Biomedical Engineering, Boston University, Boston, MA, USA.
Zackary M TicktinBiodesign Center for Molecular Design and Biomimetics at the Biodesign Institute, Arizona State University, Tempe, AZ, USA.
Soma ChaudharyBiodesign Center for Molecular Design and Biomimetics at the Biodesign Institute, Arizona State University, Tempe, AZ, USA.
Duo MaBiodesign Center for Molecular Design and Biomimetics at the Biodesign Institute, Arizona State University, Tempe, AZ, USA.
Griffin McCutcheonDepartment of Biomedical Engineering, Boston University, Boston, MA, USA.
Yudan LiMolecular Biology, Cell Biology & Biochemistry Program, Graduate School of Arts and Sciences, Boston University, Boston, MA, USA.
Kaiyue WuMolecular Biology, Cell Biology & Biochemistry Program, Graduate School of Arts and Sciences, Boston University, Boston, MA, USA.
Sanchari SahaBiodesign Center for Molecular Design and Biomimetics at the Biodesign Institute, Arizona State University, Tempe, AZ, USA.
Jonathan Alcantar-FernandezInnovation and Research Department, Salud Digna, Culiacan, Sinaloa, Mexico.
Jose L Moreno-CamachoInnovation and Research Department, Salud Digna, Culiacan, Sinaloa, Mexico.
Abraham Campos-RomeroInnovation and Research Department, Salud Digna, Culiacan, Sinaloa, Mexico.
James J CollinsDepartment of Biological Engineering, Massachusetts Institute of Technology (MIT), Cambridge, MA, USA.
Peng YinWyss Institute for Biologically Inspired Engineering, Harvard University, Boston, MA, USA.
Alexander A GreenDepartment of Biomedical Engineering, Boston University, Boston, MA, USA.

Funding

Rapid Low-Cost Paper-based Biodosimetry that reveals individual organ injuriesU01AI148319 · NIAID · ARIZONA STATE UNIVERSITY-TEMPE CAMPUS · PI LABAER, JOSHUA · 2020 to 2024
$2.5M
Molecular Fuses for Real-Time, Label-Free, Multiplexed Imaging of RNAs in Living CellsDP2GM126892 · NIGMS · BOSTON UNIVERSITY (CHARLES RIVER CAMPUS) · PI GREEN, ALEXANDER ARTHUR · 2017 to 2017
$2.3M
Programmable electrochemical gene circuit sensors for infectious disease detectionR21AI136571 · NIAID · UNIVERSITY OF TORONTO · PI GREEN, ALEXANDER ARTHUR, KELLEY, SHANA O · 2018 to 2019
$300k
NIAID NIH HHS R21 AI136571NIAID NIH HHS U01 AI148319NIGMS NIH HHS DP2 GM126892
6 · The paper itself

Abstract

Rapid, simple, and low-cost diagnostic technologies are crucial tools for combatting infectious disease. Here, we describe a class of aptamer-based RNA switches called aptaswitches that recognize specific target nucleic acid molecules and respond by initiating folding of a reporter aptamer. Aptaswitches can detect virtually any sequence and provide a fast and intense fluorescent readout, generating signals in as little as 5 minutes and enabling detection by eye with minimal equipment. We demonstrate that aptaswitches can be used to regulate folding of six different fluorescent aptamer/fluorogen pairs, providing a general means of controlling aptamer activity and an array of different reporter colors for multiplexing. By coupling isothermal amplification reactions with aptaswitches, we reach sensitivities down to 1 RNA copy/

Identifiers

PMID37333364
PMCPMC10275000

What OpenQuestion holds

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LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.